葡萄糖对奶牛乳腺上皮细胞增殖、葡萄糖摄取和酪蛋白合成相关基因表达的影响

Effects of Glucose on Cell Proliferation,Glucose Uptake and Expression of Casein Synthesis⁃Related Genes in Mammary Epithelial Cells of Dairy Cows

本试验旨在研究添加不同浓度葡萄糖对奶牛乳腺上皮细胞(BMECs)增殖、葡萄糖摄取和酪蛋白合成相关基因表达的影响。将中国荷斯坦奶牛BMCEs纯化培养后,选取第2代细胞,采用单因子完全随机设计,设置4个组,分别向培养基中添加0(对照)、5、10、20 mmol/L葡萄糖,每组3个重复。结果表明:1)与对照组相比,添加葡萄糖能显著提高BMCEs相对增殖率(P<0.05)。2)与对照组相比,10 mmol/L组BMECs的葡萄糖相对摄取率显著提高(P<0.05)。3)与对照组相比,10~20 mmol/L组BMECs的葡萄糖转运载体1(GLUT1)、葡萄糖转运载体8(GLUT8)、葡萄糖转运载体(GLUT12)的基因表达量显著上调(P<0.05)。4)与对照组相比,10 mmol/L组αs1-酪蛋白(CSN1S1)、κ-酪蛋白(CSN3)、哺乳动物雷帕霉素靶蛋白(mTOR)、真核翻译启始因子4E结合蛋白1(4EBP1)、真核翻译启始因子4E(eIF4E)和核糖体p70s6激酶(S6K1)的基因表达量显著上调(P<0.05)。综上,添加10~20 mmol/L葡萄糖能够促进BMECs对葡萄糖的摄取,上调酪蛋白合成相关基因的表达。

The aim of this study was to investigate the effects of different concentrations of glucose on the pro⁃liferation,glucose uptake and the expression of genes related with casein synthesis in bovine mammary epitheli⁃al cells(BMECs).BMECs from Chinese Holstein cows were culture and purification.A single factor random⁃ized trial design was applied.Different concentrations[0(control),5,10 and 20 mmol/L]of glucose were added in culture medium.Each group had 3 replicates.The results showed as follows:1)compared with the control group,adding glucose significantly increased the relative proliferation rate of BMCEs(P<0.05).2)Compared with the control group,the glucose related uptake rate of BMECs in 10 mmol/L group significantly promoted(P<0.05).3)Compared with the control group,the gene expression of glucose transporter 1,glu⁃cose transporter 8 and glucose transporter 12 in 10 to 20 mmol/L groups significantly up⁃regulated(P<0.05).4)Compared with the group,theαs1⁃casein,κ⁃casein,rapamycin target molecule,eukaryotic translation ini⁃tiation factor 4E binding protein,eukaryotic initiation factor 4E and ribosomal p70s6 gene expression in 10 mmol/L group significantly up⁃regulated(P<0.05).Combined with the above results,the addition of 10 to 20 mmol/L glucose can promote the uptake of glucose in BMECs,and up⁃regulate the expression of genes re⁃lated with casein synthesis.