摘要
目的:探讨miR-590-5p在卵巢癌组织中的表达情况以及其对卵巢癌细胞增殖的影响及作用机制。方法:Real-time PCR和双荧光素酶实验确定miR-590-5p与lncRNA SNHG1之间的调控作用。Real-time PCR检测卵巢癌、癌旁组织以及卵巢癌细胞中miR-590-5p的表达。分别采用NC mimic或者miR-590-5p mimic转染两株卵巢癌细胞,CCK-8检测各组细胞的增殖情况。此外,Real-time PCR检测两株细胞中SOX2、RECK和YAP1的表达。结果:Real-time PCR结果显示下调SNHG1后miR-590-5p的表达显著升高。双荧光素酶结果显示转染miR-590-5p mimic和野生型SNHG1片段的细胞中荧光素酶的活性显著降低。此外,Real-time PCR结果显示miR-590-5p在卵巢癌组织中的表达水平显著低于癌旁组织。CaOV3、OV-90细胞中miR-590-5p的表达水平明显低于其他卵巢癌细胞。转染miR-590-5p mimic显著上调了CaOV3、OV-90细胞中miR-590-5p的表达并且抑制了这两株细胞的增殖,同时抑制了两株细胞中SOX2、RECK和YAP1的表达。结论:miR-590-5p的低表达与卵巢癌的进展密切相关,miR-590-5p能够介导SNHG1信号并且通过对其下游靶基因的调控抑制卵巢癌细胞的增殖。
Objective:To investigate the expression of miR-590-5p in ovarian cancer tissue and its effect on ovarian cancer cell proliferation and its mechanism.Methods:Real-time PCR and double luciferase experiments confirmed the regulatory effect between miR-590-5p and lncRNA SNHG1.Real-time PCR was used to detect the expression of miR-590-5p in ovarian cancer tissues,adjacent tissues and ovarian cancer cells.NC mimic or miR-590-5p mimic were used to transfect two ovarian cancer cells respectively,and CCK-8 was used to detect the proliferation of each group of cells.In addition,Real-time PCR detected the expression of SOX2,RECK and YAP1 in the two cell lines.Results:Real-time PCR showed that miR-590-5p expression was significantly increased after SNHG1 was downregulated.Dual-luciferase results showed that luciferase activity in cells transfected with miR-590-5p mimic and wild-type SNHG1 fragments was significantly reduced.In addition,the Real-time PCR results showed that the expression level of miR-590-5p in CaOV3 and OV-90 cells was significantly lower than that in other ovarian cancer cells.Transfection of miR-590-5p mimic significantly increased the expression of miR-590-5p in CaOV3 and OV-90 cells and significantly inhibited the proliferation of the two cells,while inhibiting the expression of SOX2,RECK and YAP1 in two cells.Conclusion:The low expression of miR-590-5p is closely related to the progression of ovarian cancer.miR-590-5p can mediate SNHG1 signal and inhibit the proliferation of ovarian cancer cells by regulating its downstream target genes.
作者
宋洋
任芳
Song Yang;Ren Fang(Shengjing Hospital Affiliated to China Medical University,Liaoning Shenyang 110000,China)
出处
《现代肿瘤医学》
CAS
2020年第14期2365-2369,共5页
Journal of Modern Oncology
基金
国家自然科学基金青年科学基金项目(编号:81501235)。