高危型人乳头瘤病毒感染与宫颈病变患者TLR4、TRAF6、IL-12水平的关系

Relationships between high-risk human papillomavirus infection with TLR4,TRAF6,and IL-12 in patients with cervical lesions

目的:探究高危型人乳头瘤病毒(hr-HPV)感染与宫颈病变患者宫颈灌洗液和血清Toll样受体4(TLR4)、肿瘤坏死因子受体相关因子6(TRAF6)、白细胞介素12(IL-12)的关系。方法:选取我院收治的117例hr-HPV阳性宫颈病变者作为研究对象,将患者分为子宫颈上皮内瘤变(CIN)Ⅰ组35例、CINⅡ组22例、CINⅢ组29例、宫颈癌组31例,同期选取30例女性体检健康者为对照组;检测各组hr-HPV病毒载量及宫颈灌洗液和血清中TLR4、TRAF6、IL-12表达水平。结果:五组hr-HPV病毒载量比较差异具有统计学意义,组间比较均具有统计学意义(P<0.01),hr-HPV病毒载量随病变程度加重而升高;五组宫颈灌洗液TLR4、TRAF6、IL-12水平差异具有统计学意义,组间比较均具有统计学意义(P<0.01),TLR4、TRAF6水平随病变程度加重而升高,IL-12水平则降低;五组血清TLR4、TRAF6、IL-12水平差异具有统计学意义,组间比较均具有统计学意义(P<0.01),TLR4、TRAF6水平随病变程度加重而升高,IL-12水平则降低;hr-HPV感染者宫颈灌洗液与血清中TLR4、TRAF6水平同hr-HPV病毒载量呈正相关,IL-12水平同hr-HPV病毒载量呈负相关(P<0.05)。结论:hr-HPV感染患者宫颈灌洗液和血清中TLR4、TRAF6高水平表达,IL-12低水平表达,三者存在一定相关性,且与病毒载量关系密切,推测三者可用作评估宫颈病变严重程度的参考指标。

Objective:To explore the relationship between high-risk human papillomavirus(hr-HPV)infection and Toll-like receptor 4(TLR4),tumor necrosis factor receptor-related factor 6(TRAF6),interleukin-12(IL-12)in cervical blood and serum of patients with cervical lesions.Methods:117 cases of hr-HPV positive cervical lesions in our hospital were selected as the research object.The patients were divided into three groups:cervical intraepithelial neoplasia(CIN)group I(35 cases),CIN groupⅡ(22 cases),CIN groupⅢ(29 cases)and cervical cancer group(31 cases),meanwhile choosing 30 cases of female health checkers as the control group.The levels of Hr-HPV viral load,TLR4,TRAF6 and IL-12 in cervical fluid and serum were detected.Results:There were significant differences in hr-HPV viral load among the five groups(P<0.01),and the viral load of hr-HPV increased with the severity of the disease.The levels of TLR4,TRAF6 and IL-12 in cervical fluid of the five groups had statistical significance(P<0.01),and the levels of TLR4 and TRAF6 increased with the severity of cervical lesions,while the levels of IL-12 decreased.The serum levels of TLR4,TRAF6 and IL-12 in the five groups were significantly different,and there were significant differences among the groups(P<0.01).The levels of TLR4 and TRAF6 increased with the severity of the lesion,while the levels of IL-12 decreased.Cervical fluid and serum TLR4,TRAF6 were positively correlated with the viral load of hr-HPV,while IL-12 was negatively correlated with the viral load of hr-HPV(P<0.05).Conclusion:TLR4 and TRAF6 patients with hr-HPV in the cervical fluid and serum had high expression levels,and IL-12 had low expression level with some degree of correlation among the three and closely relation to viral load,speculating that the three factors can be used as the reference role for evaluating cervical lesions.