N-异丙基丙烯酰胺类纳米水凝胶在生物识别中的研究进展

Research progress of N-isopropylacrylamide hydrogel nanoparticles in recognition of biomolecule

智能型纳米水凝胶是一种内部为三维网络结构且能稳定分散在水中形成胶体体系,粒径在1~1000 nm,能感知外界环境刺激,并对其进行响应的新型高分子材料。其中具有温敏性的N-异丙基丙烯酰胺类纳米水凝胶是目前水凝胶研究的热点之一,它会随着外界温度的改变而发生体积相转变,凭借其良好的生物相容性和灵敏的温度响应性,在给药系统、蛋白质分离、医学诊断、生物传感器、生物材料等生物医学领域受到了广泛的关注。结合本课题组近年来的相关工作,主要综述了基于N-异丙基丙烯酰胺类纳米水凝胶微球在蛋白识别、药物输送与释放、DNA分离等生物识别中的应用研究和发展前景。

Hydrogel nanoparticles with a three-dimensional network structure of particle size 1~1000 nm can be stably dispersed in water to form a colloid system.Due to their stimulus responsiveness,they are often referred to as intelligent hydrogel.Among the numerous types of intelligent hydrogel,N-isopropylacrylamide nanohydrogel with thermosensitivity was further studied both on its properties and its application recently.Based on its good biocompatibility,sensitive temperature response and a volume phase change along with the change of external temperature,it was widely applied in biomedical fields such as drug delivery systems,protein separations,medical diagnostics,biosensors,and biomaterials.In this study,the application research and development prospects of N-isopropylacrylamide-based nanohydrogel microspheres in protein recognition,drug delivery and release,and DNA separation were summarized.