四逆散对FD大鼠十二指肠杯状细胞及MUC2的影响

Effects of Sini Powder on Duodenum Goblet Cells and MUC2

目的:探讨四逆散对功能性消化不良(FD)大鼠十二指肠杯状细胞及黏蛋白2(MUC2)表达的影响。方法:将30只SD大鼠随机分为正常组、模型组及四逆散观察组,每组10只。采用幼年碘乙酰胺灌胃联合夹尾应激刺激方法诱导FD动物模型,正常组及模型组给予生理盐水灌胃,四逆散观察组予四逆散中药水煎剂灌胃。取大鼠局部十二指肠组织进行HE染色观察以十二指肠组织基本形态,AB-PAS染色观察杯状细胞数量情况,免疫组织化学(IHC)观察MUC2蛋白表达情况,定量PCR(qPCR)检测MUC2 mRNA表达情况。结果:HE染色显示3组大鼠十二指肠基本形态未见明显异常;与正常组比较,模型组大鼠十二指肠杯状细胞数量明显减少;MUC2 mRNA及免疫阳性表达均明显低于正常组,差异有统计学意义(P<0.05);与模型组比较,四逆散观察组以上指标均较模型组明显升高,差异有统计学意义(P<0.05)。结论:四逆散可能通过增加FD大鼠杯状细胞数量和增加MUC2的表达,改善十二指肠黏液屏障,从而对FD起到治疗作用。

Objective:To investigate the effects of Sini Powder on goblet cells and the expression of mucin 2(MUC2)in the duodenum of functional dyspepsia(FD)rats.Methods:A total of 30 SD rats were randomly divided into 3 groups with 10 rats in each group,including a control group,a model group and a Sini Powder treatment group.The FD animal model was induced by intragastric juvenile iodoacetamide gavage combined with tail clip stress stimulation.Normal group and model group were given intragastric saline.The Sini Powder treatment group rats was given Chinese medicine decoction of Sini Powder for intragastric administration.The local duodenal tissue of rats was taken for HE staining to observe the basic morphology of the duodenal tissue,for AB-PAS staining to observe the number of goblet cells,for immunohistochemistry(IHC)to observe the expression of MUC2 protein,for quantitative PCR(qPCR)detection of MUC2 mRNA expression.Results:HE staining showed that the basic morphology of the duodenum of rats was normal in the 3 groups.Compared with the normal group,the number of duodenal goblet cells in the model group was significantly reduced;MUC2 mRNA and immunopositive expression were significantly lower than the control group(P<0.01,P<0.01),the difference was statistically significant.Compared with the model group,the above indexes of the Sini Powder treatment group were significantly higher than the model group(P<0.01,P<0.05),the difference was statistically significant.Conclusion:Sini Powder can restore mucus barrier by increasing the number of goblet cells in duodenum,regulating the expression of MUC2 so as to treat FD.