改良法制作兔右冠状动脉缺血再灌注损伤模型

Modified rabbit model of right coronary artery ischemia-reperfusion injury

目的探讨兔右冠状动脉穿线结扎后放松建立兔右冠状动脉缺血再灌注损伤模型的可行性。方法新西兰大白兔20只,雌雄不限,体重(2.5±0.3)kg,分为两组,缺血再灌注组14只,假手术组6只。假手术组右冠状动脉只穿线不结扎,另一组通过预置缝线结扎30 min后松开线结构建兔右冠状动脉缺血再灌注损伤模型。记录心电图情况、结扎前后肌酸激酶同功酶变化,术后软管插入离体心右冠状动脉验证结扎效果。结果ST段明显抬高、软管插入右冠状动脉不易拉出并肌酸激酶同功酶升高8例,制模成功率57%(8/14)。假手术组上述指标无明显变化。结论本实验改良制作右冠状动脉缺血再灌注模型,造模成功。

Objective To explore the feasibility of a simple rabbit model of right coronary artery ischemia-reperfusion injury by threading ligation.Methods 20 New Zealand white rabbits,male or female,weighing(2.5±0.3)kg,were divided into two groups:an ischemia-reperfusion group(14 rabbits)and a sham operation group(6 rabbits).Threading without ligation were treated in the sham operation group,a model of rabbit right coronary artery ischemia-reperfusion injury was constructed after loosening the thread of30 min preset suture ligation.The electrocardiogram was recorded,the changes of creatine kinase isoenzyme(CK-MB)before and after ligation,and the condition of postoperative hose insertion into the right crest were compared.Results The ST segment significantly elevated,the hose inserted into the right coronary artery was difficult to drag out and the creatine kinase isoenzyme increased in 8 cases.The success rate of the model was 57%(8/14).There were no significant changes among the above indicators in the sham operation group.Conclusions This study explores the model of right coronary artery ischemia-reperfusion-reperfusion injury,and the modeling is basically successful.