噬菌体分离纯化技术研究进展

Research Progress on Techniques for Separation,Purification of Bacteriophages

噬菌体应用领域十分广泛,因此在制备噬菌体过程中,需采用不同的技术或几种技术相结合的方法来获得具有不同纯度的噬菌体制剂。常用的技术主要包括沉淀、过滤和离心。近年来,色谱技术、场流分流技术和电泳技术等的应用,为制备噬菌体制剂提供了新的方向。

Bacteriophage is widely used in many fields,so in the process of preparing phage,the different techniques or several techniques to obtain phage preparations with different purity to be used.The techniques commonly used to separate and purify phage are precipitation,filtration and centrifugation.In recent years,the application of chromatographic,field-flow fractionation techniques and electrophoresis techniques provides a new direction for obtaining phage preparations.