基于UHPLC-PDA-Q-TOF/MS^E技术的栽培淫羊藿质量研究

Evaluation of cultivated and wild Herba Epimedii using UHPLC-PDA-Q-TOF/MS^E

采用UHPLC-PDA-Q-TOF/MS^E通过定性和相对定量方法对栽培箭叶淫羊藿、天平山淫羊藿、柔毛淫羊藿进行质量研究。本文以箭叶、天平山、柔毛淫羊藿的栽培种质、相应野生种质以及来自国内其他野生种质为研究对象,采用UHPLC-Q-TOF/MS^E结合UNIFI数据分析平台快速鉴定淫羊藿中化合物32个,同时利用主成分分析(PCA)法对3个种栽培及野生样本进行比较,结果表明栽培与相应野生样本化学成分组成比较一致。最后利用UHPLCPDA对所有样本中12个黄酮类成分及总黄酮进行相对定量,结果表明3个种的栽培与相应野生种质化学成分相对含量较相似,且栽培后淫羊藿质量稳定性较好。本文利用UHPLC-PDA-Q-TOF/MS^E结合UNIFI数据分析平台和PCA方法建立的定性和相对定量方法可用于栽培淫羊藿的质量研究,同时研究结果也为淫羊藿药材的栽培推广和资源合理开发利用提供了科学依据。

Qualitative and relatively quantitative methods were used to study the quality of cultivated Epimedium sagittatum(Sieb. et Zucc.) Maxim., Epimedium myrianthum Stearn, and Epimedium pubescens Maxim by ultra high-performance liquid chromatography coupled with photo-diode array and quadrupole time-of-flight mass spectrometry(UHPLC-PDA-Q-TOF/MS^E). Thirty-two compounds in cultivated and wild samples of E. sagittatum,E. myrianthum and E. pubescens were identified using UHPLC-Q-TOF/MS^E combined with the UNIFI data analysis platform. Principal component analysis(PCA) was used to compare the cultivated and wild samples of these three species. The results show that the chemical compositions of cultivated samples were consistent with the corresponding wild samples. UHPLC-PDA was used to determine the relative content of 12 flavonoids as well as total flavonoids in all samples. The results show that the relative chemical content of these flavonoids in cultivated and wild samples is similar and the quality of cultivated Epimedium is more stable. These qualitative and relatively quantitative methods using UHPLC-PDA-Q-TOF/MS^E combined with the UNIFI data analysis platform and PCA can be used to study the quality of cultivated Herba Epimedii. This research provides a scientific basis for the cultivation and rational development and utilization of Epimedium medicinal materials.