禽网状内皮组织增生病病毒感染对SPF雏鸡血液和局部淋巴组织CD4^+/CD8^+细胞及相关细胞因子表达的影响

Effects of Avian Reticuloendotheliosis Virus Infection on the CD4^+/CD8^+Ratio and the Expression of Related Cytokines in SPF Chicks

旨在探讨禽网状内皮组织增生病病毒(reticuloendotheliosis virus,REV)对SPF雏鸡血液和局部淋巴组织中T淋巴细胞数量以及相关细胞因子表达的影响。将96只1日龄SPF雏鸡随机分为REV感染组和对照组,应用流式细胞术、酸性α-醋酸萘酯酶染色(acidα-naphthyl acetate esterase,ANAE)、荧光定量PCR等方法对上述指标进行检测。试验数据表明,与对照组相比,感染组雏鸡血液CD4^+T淋巴细胞数量在第7-35天显著降低、CD8^+T淋巴细胞数量在第7-28天显著升高,CD4^+/CD8^+比值在第7-28天显著降低(均P<0.05或P<0.01);局部淋巴组织哈德尔腺(Hader’s gland,HG)、派伊尔结(Peyer’s patch,PP)和盲肠扁桃体(caecal tonsil,CT)中ANAE^+T淋巴细胞数量均显著降低(均P<0.05或P<0.01);GH、PP和CT中细胞因子IL-2、IFN-γ和TNF-α转录量都有不同程度升高。本研究表明,REV感染引起雏鸡血液中CD4^+T淋巴细胞数量降低、CD4^+/CD8^+T淋巴细胞比例失衡、局部淋巴组织中T淋巴细胞数量相对减少及细胞因子TNF-α转录持续升高与REV造成感染雏鸡细胞免疫功能显著降低密切相关。

The study aimed to investigate the effects of avian reticuloendotheliosis virus(REV)on the number of T lymphocytes in blood and the expression of related cytokines in local lymphoid tissues of SPF chicks.Ninety-six 1-day-old SPF chicks were randomly divided into REV-infected group and control group.The above indexes were detected by Flow Cytometry,acidα-naphthyl acetate esterase(ANAE)and Quantitative Real-time PCR.The results showed that compared with the control group,the number of CD4^+T lymphocytes in the infected group was significantly decreased at day 7-35,and the number of CD8^+T lymphocytes was significantly increased at day 7-28,the CD4^+/CD8^+ratio was significantly decreased at day 7-28(all P<0.05 or P<0.01);the number of ANAE^+T lymphocytes in the local lymphoid tissues of Hader’s gland(HG),Peyer’s patch(PP)and caecal tonsil(CT)were significantly decreased(all P<0.05 or P<0.01);the transcription levels of cytokines IL-2,IFN-γand TNF-αin HG,PP and CT were increased in various degrees.These data indicated that REV infection caused a decrease in the number of CD4^+T lymphocytes in the peripheral blood of chickens,an imbalance of the ratio of CD4^+/CD8^+T lymphocytes,a relative decrease in the number of T lymphocytes in local lymphoid tissues,and a continuous increase in the expression of cytokines TNF-αare closely related to the significantly reduced cellular immune function of infected chickens caused by REV.