扶正解毒方抗肺癌作用生物信息分析

Bioinformatics analysis of the Fuzheng Jiedu prescription against lung cancer

目的:利用生物信息分析挖掘扶正解毒方抗肺癌作用的分子机制。方法:利用BATMAN生物信息平台和GeneCards数据库筛选出扶正解毒方人肺癌相关靶基因。利用STRING数据库、KEGG数据库及Cytoscape软件对相关靶基因进行GO分析、信号通路分析,挖掘出该方抑瘤作用的主要生物功能及信号通路。结果:GO分析提示,扶正解毒方人肺癌相关靶基因在信息传递、调节程序性死亡、调节免疫等方面聚类显著。信号通路分析显示富集在磷脂酰肌醇三激酶/丝氨酸(PI3K/AKT)、缺氧诱导因子(HIF-1)、细胞凋亡(Apoptosis)、肿瘤坏死因子(TNF)、有丝分裂原激活的蛋白激酶(MAPK)等通路。结论:扶正解毒方可能通过调节细胞程序性死亡、调节免疫、血管生成及细胞黏附、迁移等多种途径发挥作用,其中最为主要的作用途径为促进细胞程序性死亡和凋亡,其关键信号通路可能为PI3K/AKT信号通路。

Objective:Exploring the possible molecular mechanism of the Fuzheng Jiedu prescription against lung cancer by using bioinformatics analysis.Methods:The BATMAN-ATM and GeneCards database were used to screen out the target genes related to lung cancer in the Fuzheng Jiedu prescription.The STRING database,KEGG database and Cytoscape software were used to analyze the target genes for GO analysis,signal pathway.Results:GO analysis suggested that clustering was significant in information transmission,regulation of programmed death,regulation of immunity.Signal pathway enrichment in PI3K/AKT,HIF-1,Apoptosis,TNF,MAPK and etc..Conclusion:The Fuzheng Jiedu prescription may play a role in regulating cell death,regulating immunity,angiogenesis,cell adhesion,migration,etc,and the more important roles are regulating cell death.Inducing apoptosis,may via PI3K-AKT passway.