低强度纳秒脉冲电场联合靶向金纳米棒对A375黑色素瘤细胞的杀伤效果研究

Study of Killing Effects of A375 Melanoma Cells Treated by Low Intensity Nanosecond Pulsed Electric Fields Combined with Targeted Gold Nanorods

为了改善纳秒脉冲电场(nsPEFs)在治疗肿瘤过程中存在的电气安全性问题,该文首次研究低强度nsPEFs联合叶酸修饰的金纳米棒对A375黑色素瘤细胞杀伤效果的影响。首先对聚乙二醇-金纳米棒(GNR-PEG)的表面进行叶酸(FA)的修饰,从而实现对A375黑色素瘤细胞的靶向作用;然后用暗场显微镜观察其结合效果。该文在确定了GNR-PEG和GNR-PEG-FA安全质量分数后,通过改变电场强度(2~8kV/cm)和脉冲个数(15~260个)来研究该联合方法对细胞活性和凋亡的影响。结果发现,GNR-PEG-FA与低强度nsPEFs联合显示出最佳的抗肿瘤效果,在较低的电场强度和较少的脉冲个数作用下,实现了更低比例的细胞的活性和更高比例的细胞凋亡。该联合方法增强了nsPEFs对A375黑色素瘤细胞杀伤效果,改善了nsPEFs治疗中的电气安全性问题。

To improve the electrical safety associated with nanosecond pulsed electric fields(nsPEFs)in tumor treatment,for the first time,the killing effects of low-intensity nsPEFs combined with folic acid(FA)modified gold nanorods on A375 melanoma cells were investigated.The surface of polyethylene glycol-gold nanorods(GNR-PEG)was modified by folic acid(GNR-PEG-FA)to target A375 melanoma cells,and the binding efficacy was observed by dark field microscopy.After determining the safe concentrations of GNR-PEG and GNR-PEG-FA,the effects of the combined treatment on cell viability and apoptosis were studied by changing electric field strengths(2~8kV/cm)and pulse numbers(15~260).It is found that the combination of low-intensity nsPEFs and GNR-PEG-FA shows the best antitumor efficacy,resulting in lower percentages of viable cells and higher percentages of apoptosis at lower electric field strengths and fewer pulses.This combined treatment enhances the killing effects of A375 melanoma cells and improves the electrical safety of nsPEFs treatment.