HIF-1α和COX-2在溃疡性结肠炎患者外周血及黏膜组织中的表达及临床意义

Expression and clinical significance of HIF-1αand COX-2 in peripheral blood and mucosal tissues of patients with ulcerative colitis

目的探讨低氧诱导因子-1α(HIF-1α)和环氧化酶-2(COX-2)在溃疡性结肠炎患者外周血及黏膜组织中的表达及临床意义。方法选取2019年1~6月在茂名市茂南区人民医院及广东省人民医院内科收治的50例溃疡性结肠炎患者为研究对象,按组织学进行分组,将溃疡性结肠炎组织作为组织实验组,将同一患者正常结肠组织(溃疡周边组织)作为组织对照组;按血清学进行分组,将50例溃疡性结肠炎患者作为血清实验组,将50例健康体检者作为血清对照组;分别检测血清和结肠黏膜中HIF-1α和COX-2水平的表达,并分析两种指标与临床病理学参数间的关系。结果组织实验组的HIF-1α和COX-2平均阳性细胞数高于组织对照组,差异均有统计学意义(P<0.05);血清实验组的HIF-1α和COX-2水平高于血清对照组,差异均有统计学意义(P<0.05);黏膜组织中的HIF-1α、COX-2水平与临床病情严重程度及内镜分级情况成正相关(r=0.812、0.758、0.829、0.764,P<0.05);血清中的HIF-1α、COX-2水平与临床病情严重程度及内镜分级情况成正相关(r=0.801、0.749、0.812、0.758,P<0.05)。结论 HIF-1α和COX-2在溃疡性结肠炎患者外周血及黏膜组织中的表达明显升高,与病情严重程度成正相关,为临床诊断及临床治疗评估提供了可靠依据。

Objective To investigate the expression and clinical significance of hypoxia-inducible factor-1α(HIF-1α)and cyclooxygenase-2(COX-2)in peripheral blood and mucosal tissues of patients with ulcerative colitis.Methods From January to June in 2019,50 patients with ulcerative colitis who were admitted to the Department of Internal Medicine of People′s Hospital of Maonan District of Maoming City and Guangdong Provincial People′s Hospital were selected as the research subjects.According to histological classification,ulcerative colitis tissue were categorized into the experimental tissue group,and normal colon tissues from the same patients(surrounding ulcerative tissue)were used as the tissue control group.Based on the classification of serology,serum from 50 patients with colitis were classified into the experimental serum group,and serum from the 50 healthy subjects were classified into the serum control group.The expression of HIF-1αand COX-2 in the serum and colonic mucosa were detected,and relationships between two indicators and clinical pathological parameters were analyzed.Results The average number of HIF-1αand COX-2 positive cells in the experimental tissue group were higher than those in the control tissue group,with statistical significance(P<0.05).The levels of HIF-1αand COX-2 in the experimental serum group were higher than those in the control serum group,and the differences were statistically significant(P<0.05).The levels of HIF-1αand COX-2 in mucosal tissues were positively correlated with clinical severity and endoscopic grade(r=0.812,0.758,0.829,0.764,P<0.05).The level of HIF-1αand COX-2 in serum were positively correlated with clinical severity and endoscopic grade(r=0.801,0.749,0.812,0.758,P<0.05).Conclusion The expression of HIF-1αand COX-2 in peripheral blood and mucosal tissues of patients with ulcerative colitis remarkably increase,which are positively correlated with the severity of the disease,providing a reliable basis for clinical diagnosis and treatment assessment.