鼠李糖乳杆菌hsryfm 1301发酵乳改善大鼠非酒精性脂肪肝特性研究

Effect of Lactobacillus rhamnosus Hsryfm 1301 Fermented Milk on the Characteris⁃tics of Nonalcoholic Fatty Liver in Rats

前期体外试验发现,鼠李糖乳杆菌hsryfm 1301经长时间发酵(72 h)可提升其益生特性;本文在前期研究的基础上,通过体内动物试验进一步验证鼠李糖乳杆菌hsryfm 1301长时发酵乳(72 h)与常规时间发酵乳(17 h)干预对非酒精性脂肪肝大鼠的益生作用;并测定了大鼠血清中血脂指标、游离脂肪酸、活性氧及炎症因子等含量。研究结果表明:72 h发酵乳干预后,大鼠血清中TC、TG以及LDL-L含量分别为2.06 mmol/L、1.47 mmol/L、1.03 mmol/L,显著低于模型组(P<0.05),HDL-L含量为0.60 mmol/L,显著高于模型组(P<0.05);17 h发酵乳组和72 h发酵乳组两者之间含量无显著性差异(P>0.05);17 h发酵乳组和72 h发酵乳组还显著降低了大鼠血清中游离脂肪酸、活性氧、TNF-α和IL-6的含量,其中,72 h发酵乳分别为1188.68 U/L、339.88 U/mL、143.11 pg/mL及112.37 pg/mL,显著低于17 h发酵乳(P<0.05)。综上,鼠李糖乳杆菌hsryfm 1301发酵乳对大鼠的非酒精性脂肪肝具有良好的改善作用,其中72 h发酵乳具有更好地抗炎、抗氧化特性,为进一步研究鼠李糖乳杆菌hsryfm 1301发酵乳益生特性提供指导意义。

It was found that Lactobacillus rhamnosus hsryfm 1301 could improve its prebiotic properties after long-term fermentation(72 h);On the basis of previous studies,the prebiotic effects of Lactobacillus rhamnosus hsryfm 1301 long-term fermented milk(72 h)and conventional fermented milk(17 h)on rats with nonalcoholic fatty liver were further verified by animal experiments in vivo,and the serum lipid index,free fatty acid,active oxygen and inflammatory factors were measured.The results showed that the contents of TC,TG and LDL-L were 2.06 mmol/L,1.47 mmol/L and 1.03 mmol/L,respectively,which were significantly lower than those of the model group(P<0.05),and the contents of HDL-L were 0.60 mmol/L,which were significantly higher than those of the model group(P<0.05),but there was no significant difference in the contents of TC,TG,HDL-L and LDL-L between the 17 h fermented milk group and the 72 h fermented milk group(P>0.05)At the same time,the contents of free fatty acid,active oxygen,TNF-αand IL-6 in serum of rats were significantly reduced,among which,the 72 h fermented milk was 1188.68 U/L,339.88 U/mL,143.11 pg/mL and 112.37 pg/mL,respectively,significantly lower than that of 17 h fermented milk(P<0.05).In conclusion,the fermented milk of Lactobacillus rhamnosus hsryfm 1301 has a good effect on the improvement of non-alcoholic fatty liver in rats,and the 72 h fermented milk has better anti-inflammatory and anti-oxidation characteristics,which provides guidance for further study on the probiotic characteristics of the fermented milk of Lactobacillus rhamnosus hsryfm 1301.