摘要
结合环介导等温扩增技术(Loop-mediated isothermal amplification,LAMP),PMA/EMA-LAMP在高灵敏度、特异性和快速性方面明显优于传统的基于PCR的检测方法。特别是PMA/EMA-LAMP在VBNC状态下对大肠埃希菌、副溶血性弧菌和葡萄球菌的检测已被证明是一种有效的方法。本文综述了此方法的应用及作用机理。
Combined with loop-mediated isothermal amplification(LAMP),PMA/EMA-LAMP is superior to traditional PCR based detection methods in high sensitivity,specificity and rapidity.In particular,PMA/EMA-LAMP has been proved to be an effective method for the detection of Escherichia coli,Vibrio Parahaemolyticus and Staphylococcus in VBNC.This paper reviews the application and mechanism of this method.
作者
冯雪
王冠蕾
侯俊材
FENG Xue;WANG Guanlei;HOU Juncai(Key Laboratory of Dairy Science,Ministry of Education,Northeast Agricultural University,Harbin 150030,China;Cheng de Petroleum College,Chengde 067000,China)
出处
《中国乳品工业》
CAS
北大核心
2020年第5期37-41,共5页
China Dairy Industry
基金
“十三五”国家重点研发计划(2016YFD0400605)。
关键词
乳制品
非可培养态
食源性致病菌
环介导等温扩增
Dairy products
Non-culture
Foodborne pathogens
Loop-mediated isothermal amplification
