红花CtGATA21基因的克隆及原核表达分析

Cloning and prokaryotic expression of CtGATA21 gene in safflower

植物在整个生命周期中会遇到各种各样的非生物胁迫,而转录因子在植物响应非生物胁迫过程中发挥重要功能。GATA是一类能够参与细胞分化、生长发育和响应胁迫的转录因子,目前还没有广泛的研究。本试验从菊科植物红花中克隆并获得了一个B类GATA基因(命名为Ct GATA21, NCBI登录号:MN399373)。实验结果显示CtGATA21基因全长966 bp,编码321个氨基酸,理论分子量为36.02 kDa,且定位于细胞核中。进一步分析发现,红花CtGATA21的表达具有组织特异性,在叶片中表达量最高;而在盐、干旱胁迫下其表达量迅速积累,显著上调,表明红花CtGATA21能够正响应盐和干旱胁迫,这可能为进一步分析植物GATA转录因子的抗逆功能提供了新的数据。

Plants encounter various abiotic stresses during the whole life cycle. The transcription factors act as the key roles in response to stresses. GATA transcription factors are involved in cell differentiation, growth and development and responding to stresses, which have not widely been studied so far. In the present study, a group B GATA gene(named as CtGATA21, accession number: MN399373) was isolated from safflower in Compositae, which was 966 bp long and encoded 321 putative amino acids with an estimated molecular weight of 36.02 kDa and localized to the nucleus. Further quantitative RT-PCR analysis indicated that the expression of CtGATA21 had tissue specificity and significantly accumulated and up-regulated under salt and drought stress. In conclusion, our results indicate that CtGATA21 is a positive regulator in response to salt and drought stress in safflower, which might provide new data for further analyzing the function of plant GATA transcription factors.