AhATL1异源表达拟南芥植株对ABA敏感性和干旱响应的变化

Changes of AhATL1 heterologous expression in Arabidopsis thaliana plants in response to ABA sensitivity and drought

Ah ATL1是花生(Arachis hypogaea) ABA转运蛋白类似基因(arachis hypogaea abscisic acid transporter like-1),其编码的蛋白具有ABCG亚家族保守功能结构域。本文对拟南芥植株、Atabcg22、AhATL1-OX进行脱落酸(ABA)敏感性和抗旱性分析,并检测了胁迫响应基因及ABA信号相关基因的表达。结果表明:ABA外施处理使Ah ATL1-OX植株的种子绿胚率和主根伸长提高,说明Ah ATL1的异源表达降低了ABA敏感性。在干旱条件下,与野生型比较, AhATL1-OX植株的叶片相对含水量降低,存活率降低,体内ABA含量降低,胁迫响应基因RD29A、RAB18与ABA信号相关基因PP2C5的表达降低,说明AhATL1的异源表达提高了干旱敏感性,与干旱条件下ABA信号通路相关基因和干旱胁迫响应基因的表达变化有关。

AhATL1 is a peanut ABA transporter like gene(Arachis hypogaea abscisic acid transporter like-1), which encodes a protein with a conserved functional domain of the ABCG subfamily. In this paper, Col, Atabcg22 and AhATL1-OX were analyzed for ABA sensitivity and drought resistance, and the expression of stress response genes and ABA signaling related genes were detected. The results showed that the ABA external application increased the seed germination rate and main root growth of AhATL1-OX plants, indicating that the heterologous expression of AhATL1 reduced ABA sensitivity. Under drought conditions, compared with Col, the relative water content of AhATL1-OX plants decreased, the survival rate decreased, the ABA content decreased, the expression of stress response genes RD29 A and RAB18 and ABA signal related genes PP2 C5, ALMT12 and SLAC1 decreased. Heterologous expression of AhATL1 increased drought sensitivity and was associated with changes in the expression of genes involved in ABA signaling pathways under drought conditions.