桦木酸对T-2毒素致小鼠肠道氧化损伤的保护作用

Protective Effect of Betulinic Acid on Intestinal Oxidative Damage Induced by T-2 Toxin in Mice

采用腹腔注射T-2毒素诱导小鼠肠道氧化损伤模型,研究桦木酸(betulinic acid,BA)对肠道氧化损伤的保护作用。60只健康雄性KM小鼠随机6组,分别为对照组,T-2毒素组,BA低、中、高剂量(0.25、0.5、1mg/kgmb)+T-2毒素组,VE+T-2毒素组。BA连续灌胃14 d后,检测血清中免疫球蛋白(immunoglobulin,Ig)G、Ig M和二胺氧化酶(diamineoxidase,DAO)活性,十二指肠、空肠和回肠等小肠组织中过氧化氢酶(catalase,CAT)和谷胱甘肽过氧化物酶(glutathioneperoxidase,GSH-Px)活性,以及谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)含量的变化,苏木精-伊红染色观察空肠形态结构的改变,探究BA对肠道氧化损伤的保护作用。结果表明:BA预处理能提高血清IgM、IgG水平,降低血清DAO活性,提高十二指肠、空肠和回肠中CAT和GSH-Px活性,升高GSH水平,降低MDA含量,缓解T-2毒素诱导的肠黏膜结构的损坏。结论:BA通过提高机体的体液免疫能力、增强肠黏膜屏障功能和提高肠道抗氧化损伤的能力,改善肠道结构的完整性,对T-2毒素引起的小鼠肠黏膜氧化损伤具有预防性的保护作用。

In order to explore the protective effect of betulinic acid (BA)on T-2 toxin-induced intestinal oxidative damage,60 healthy male mice were randomly divided into 6 equal groups:control,T-2 toxin,low-,medium-and high-dose BA(0.25,0.5 and 1 mg/kg mb)plus T-2,and vitamin E plus T2 After BA was administred for 14 days,the levels of immunoglobulin(Ig)M,IgG,and diamine oxidase (DAO)in serum were detected by enzyme linked immnosorbent assay,the activities of catalase(CAT)and glutathione peroxidase(GSH-Px),and the contents of malondialdehyde(MDA)and glutathione(GSH)in the duodenum,jejunum and ileum were determined.Morphological changes in the jejunum were observed by hematoxylin-eosin(H&E)staining.The results showed that BA enhanced the levels of IgG and IgM,and reduced the activity of DAO in the serum.Meanwhile,it alleviated the decrease of CAT and GSH Px activities caused by T-2 toxin in the intestine,increased GSH content,and decreased MDA content.Therefore,BA has a protective efect on intestinal oxidative damage induced by T-2 toxin in mice by increasing humoral imunity function,improving intestinal mucosal barrier function and enhancing intestinal antioxidant capacity.