负载姜黄素支架体内构建组织工程软骨的可行性研究

The Feasibility of Constructing Tissue Engineering Cartilage with Curcumin-Loaded Scaffold in Vivo

目的探索以负载姜黄素材料为支架,在动物皮下构建组织工程软骨的可行性。方法通过细胞划痕试验明确既可抑制血管但又不影响干细胞生物活性的姜黄素的最佳浓度范围。负载姜黄素的支架材料与单纯明胶支架材料分别植入裸鼠皮下,术后3、6周取材,比较局部皮下血管抑制程度。软骨细胞-负载姜黄素支架复合物与软骨细胞-明胶支架复合物分别植入裸鼠皮下,术后3、6周取材进行组织学检测,比较软骨形成情况。结果姜黄素对h UVEC的有效抑制浓度为30μmol/L,而对BMSC的有效抑制浓度为40μmol/L。负载姜黄素支架在裸鼠皮下具有明显的血管抑制作用。软骨细胞-负载姜黄素支架复合物在裸鼠皮下可形成成熟软骨。结论负载姜黄素支架具有血管抑制作用且不影响软骨再生,有望成为软骨再生的理想支架。

Objective To explore the feasibility of constructing tissue engineering cartilage with curcumin-loaded scaffold in vivo.Methods The optimal concentration range of curcumin that inhibits the vascularization without affecting stem cell cartilage regeneration was identified by cell scratch test.The curcumin-loaded scaffold and gelatin scaffold were implanted subcutaneously in nude mice.The materials were then harvested after 3,6 weeks and the inhibition degree of subcutaneous blood vessels was compared.Chondrocytes/curcumin-loaded scaffolds and chondrocytes/gelatin scaffolds were implanted subcutaneously in nude mice.The above constructs were then harvested 3,6 weeks after implantation for the evaluation of cartilage formation.Results The effective inhibition concentration of curcumin on HUVEC was 30μmol/L,while the effective inhibition concentration of curcuminon BMSC was 40μmol/L.The curcumin-loaded scaffold showed significant vascular inhibition in nude mice.Chondrocytes/curcumin-loaded scaffolds formed mature cartilage subcutaneously in nude mice.Conclusion The curcumin-loaded scaffold is expected to be an ideal scaffold for cartilage regeneration because of its vascular inhibition and no effect on cartilage regeneration.