荞麦蜂花粉破壁扫描电子显微镜分析及黄酮模拟消化释放

Scanning Electron Microscope Analysis of Cell Wall Breakdown of Buckwheat Bee Pollen and Release of Flavonoids during Its Simulated Gastrointestinal Digestion

以扫描电子显微镜法研究荞麦蜂花粉低温聚能超声破壁过程,发现在外加聚能超声作用下,蜂花粉破壁是沿3个萌发沟裂开,接着破裂,最后破碎的过程,据此提出电子显微镜视野中更加精准的破壁率计算方法。在扫描电子显微镜观测指导下,获得最佳的破壁条件为初始温度0℃、超声功率400 W、脉冲通断比1∶2、超声时间30 min、料液比1∶30(g/mL),在此条件下,破壁率达98.33%。进一步研究发现,破壁率与蜂花粉细胞内黄酮在模拟肠胃液中的总释放率呈线性增加关系,线性方程为y=0.3638x+27.172,R2=0.9997。

Low-temperature energy-focusing ultrasound was used to break down the cell wall of buckwheat bee pollen and the process was analyzed using scanning electron microscope(SEM).In the microscopic field,the bee pollen cells were spited,ruptured along the germ ditch,and broken into pieces successively,which allows the establishment of an accurate method for calculating the efficiency of cell wall disruption.Based on SEM observation,the optimum experimental conditions that provided the maximum efficiency of cell wall disruption(98.33%)were determined as follows:initial temperature 0℃,ultrasound power 400 W,pulse duty factor 1:2,sonication time 30 min,and ratio of solid to solvent 1:30(g/mL).Further study showed that the efficiency of cell wall disruption displayed a linear relationship with the release percentage of total flavonoids from the pollen when incubated in simulated gastric and intestinal fluid,which was fitted into the following equation was y=0.3638x+27.172 with a correlation coefficient(R2)of 0.9997.