基于Fe3O4-PGA@Au构建无酶电化学生物传感器检测葡萄糖

Fabrication of Non-enzymatic Amperometric Glucose Biosensor Based on Fe3O4-Poly-(γ-glutamic acid)@Au

基于聚谷氨酸(poly-(γ-glutamic acid),PGA)金磁微粒(Fe3O4-PGA@Au)电沉积修饰玻碳电极,构建具有高灵敏度和选择性的无酶型葡萄糖电化学生物传感器。采用绿色还原吸附法制备Fe3O4-PGA@Au,并利用X射线衍射、傅里叶变换红外光谱和透射电子显微镜表征Fe3O4-PGA@Au的理化性质。优化葡萄糖传感检测体系的反应温度、pH值、扫描速率和反应时间等检测参数。采用循环伏安法考察无酶传感器检测葡萄糖时的电化学行为。结果表明,Fe3O4-PGA@Au具有良好的电催化性能;在葡萄糖浓度分别为0.1~5.0μmol/L和10~250μmol/L范围内时,所构建的生物传感器峰电流密度与其葡萄糖浓度具有良好的线性关系,检出限为0.74μmol/L(RSN=3);此无酶型生物传感器具有好的重复性和抗干扰性能。

Herein we report a novel highly sensitive and selective non-enzymatic glucose biosensor fabricated using a Fe3O4-poly-(γ-glutamic acid)@Au(Fe3O4-PGA@Au)-modified glassy carbon electrode.By a green reduction and adsorption method,Fe3O4-PGA@Au was successfully prepared and characterized by X-ray differentiation(XRD),Fourier transform infrared(FT-IR)spectroscopy and transmission electron microscopy(TEM).The reaction temperature,pH value,scan rate and time were optimized.Cyclic voltammetry was used to investigate the biosensor performance.The results showed that Fe3O4-PGA@Au had good electrocatalysis performance.Good linearity was observed between the peak current density of the biosensor and glucose concentration in the range of 0.1–5.0 and 10–250μmol/L with a detection limit(LOD)of 0.74μmol/L(RSN=3).In addition,the biosensor showed excellent reproducibility.It could efficiently resist the interference of coexistent substances.