FCM联合多重PCR技术在慢性淋巴细胞白血病基因异常检测中的效果分析

Effect of FCM Combined with MultipleχPCR on Abnormal Gene Detection of Chronic Lymphocytic Leukemia

目的探讨FCM(流式细胞术)联合多重PCR(聚合酶链式反应)技术在慢性淋巴细胞白血病基因异常检测的效果。方法选择2016年4月至2018年4月本院收治的慢性淋巴细胞白血病患者52例作为观察组,另选择同时间段本院接收健康体检者52例作为对照组,均取外周血标本和骨髓标本,采用FCM联合多重PCR技术进行检测,比较两组T细胞各亚群细胞表达水平,分析不同特征患者IgVH基因重排阳性率。结果观察组CD45+、CD19+细胞百分率显著低于对照组,而CD34+、CD10+细胞百分率则显著高于对照组,P<0.05;不同性别、不同年龄和不同分期患者IgVH基因重排阳性率比较无显著性差异,P>0.05。结论在慢性淋巴细胞白血病诊断中采用FCM联合多重PCR技术进行检测对诊治疾病有较高价值,尤其对基因异常甲基化检出率较高,为治疗方案制定提供可靠的依据,研究价值较高。

Objective To investigate the effect of FCM(fl ow cytometry)combined with multipleχPCR(polymerase chain reaction)on the detection of genetic abnormalities in chronic lymphocytic leukemia.Methods A total of 52 patients with chronic lymphocytic leukemia admitted to our hospital from April 2016 to April 2018 were enrolled as the observation group.52 patients who received healthy physical eχamination at the same time were selected as the control group.All peripheral blood samples were taken.The bone marrow specimens were detected by FCM combined with multipleχPCR,and the eχpression levels of T cells in each group were compared.The positive rate of IgVH gene rearrangement in patients with different characteristics was analyzed.Results The percentages of CD45+and CD19+cells in the observation group were signifi cantly lower than those in the control group,while the percentages of CD34+and CD10+cells were signifi cantly higher than those in the control group,P<0.05.The weight of IgVH gene was different in different genders,ages and stages.There was no signifi cant difference in the positive rate of the row,P>0.05.Conclusion The detection of FCM combined with multipleχPCR in the diagnosis of chronic lymphocytic leukemia has a high value for the diagnosis and treatment of diseases,especially for the detection of abnormal methylation of genes,which provides a reliable basis for the development of treatment plans,higher research value.