摘要
为探究环境剂量磷酸三(1,3-二氯异丙基)酯(TDCIPP)多代暴露对生物体的影响,选取斑马鱼为模型,研究了斑马鱼暴露于0、3、30和300 ng·L^-1TDCIPP至3代后,对每一代子代5 dpf仔鱼神经发育的毒性效应。研究结果表明,F0代暴露于300ng·L^-1TDCIPP 120 d后所产F1代仔鱼的孵化率显著性下降,存活率显著性降低;但对F2代和F3代仔鱼的这些终点指标均无显著性影响。运动行为结果表明,F0代暴露于3和300 ng·L^-1TDCIPP 120 d会导致F1代仔鱼在光暗周期刺激下的游泳速度受到抑制,并伴随着神经元发育基因(ngn1)以及轴突生长标志基因(α1-tubulin、netrin1b和zn5)的显著性上调,相关性分析表明,游泳速度的抑制与ngn1、α1-tubulin和zn5这3个基因的表达显著相关。但对F2代仔鱼,仅300 ng·L^-1TDCIPP导致其游泳速度在黑暗刺激下显著性下降,且导致神经发育和再生相关基因(elavl3、gap43、gfap和shha)表达量显著性下降,但游泳速度的下降与基因表达无显著相关性。继续暴露至F3代仔鱼时,TDCIPP暴露对运动行为不再有显著影响。研究表明,环境剂量TDCIPP多代暴露对子代仔鱼具有神经发育毒性,表现为运动行为受损和神经发育相关基因表达量的改变,但毒性效应随着暴露代数的增加而减弱。
In order to investigate the multigenerational exposure effects of TDCIPP at environmental concentrations,we selected zebrafish(Danio rerio)as a model and studied the developmental neurotoxicity on 5 dpf zebrafish larvae of each generation after exposure to 0,3,30 and 300 ng·L^-1 TDCIPP for three consecutive generations.Our results showed that hatching rates and survival rates were significantly affected in the 5 dpf larvae of F1 generation,but were unaffected in 5 dpf larvae of neither F2 nor F3 generation.Behavioral measurements showed that exposure to 3 or 300 ng·L^-1 TDCIPP significantly decreased the swimming behavior response of the 5 dpf larvae of F1 generation to both light and dark stimulation.TDCIPP exposure significantly increased expression of the neural marker gene ngn1 and axon-related genes(α1-tubulin,netrin1b and zn5)in F1 larvae.In addition,correla-tion analysis showed that the inhibition of swimming speed was significantly correlated with the expression of genes,i.e.,ngn1,α1-tubulin and zn5,which suggested that the disturbance on nerve development might lead to the abnormal behaviors in F1 larvae.For F2 larvae,only 300 ng·L^-1 TDCIPP caused a significant decrease in swimming speed under dark stimulation,and resulted in a significant decrease in the expression of genes related to nerve development and regeneration(elavl3,gap43,gfap,and shha).However,there was no significant correlation be-tween the inhibition of behaviors and the altered expressions of genes in F2 larvae.For F3 larvae,TDCIPP exposure no longer had a significant effect on motor behavior.Taken together,our results showed that the multigenerational exposure to TDCIPP at environmental concentrations had neurodevelopmental toxicity to the offspring larvae,which exhibited changes in motor behavior and/or expressions of neurodevelopment-related genes,but the toxic effects decreased with the increase of number of generations being exposed.
作者
丁希胜
马徐发
余丽琴
Ding Xisheng;Ma Xufa;Yu Liqin(College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China;Hubei Provincial Engineering Laboratory for Pond Aquaculture,Wuhan 430070,China)
出处
《生态毒理学报》
CAS
CSCD
北大核心
2020年第2期50-60,共11页
Asian Journal of Ecotoxicology
基金
国家自然科学基金面上项目(21677057)
国家自然科学基金青年项目(21307162)。