血清miR-122和miR-199a对子宫内膜异位症的诊断价值分析被引量：4

Diagnostic value of serum miR-122 and miR-199a in women with endometriosis

下载PDF

导出

摘要目的评价血清miR-122和miR-199 a在诊断子宫内膜异位症的的价值。方法对2016年1月至2018年1月我院80名盆腔子宫内膜异位症患者和70名因盆腔疼痛接受腹腔镜检查但未被诊断为子宫内膜异位症的妇女进行了前瞻性队列研究。采集血液和腹膜液样本,用酶联免疫吸附法测量白细胞介素-6 (IL-6),用定量实时聚合酶链反应测量miR-122和miR-199a表达量。结果子宫内膜异位症患者血清和腹膜液中的IL-6、miR-122和miR-199a水平明显高于对照组,差异有统计学意义(P<0.001)。血清miR-122的表达与血清IL-6 (r=0.591),血清miR-199a (r=0.746),腹膜液IL-6 (r=0.611),腹膜液miR-122 (r=0.956),腹膜液miR-199a(r=0.678)呈正相关。同时,血清miR-199 a的表达与血清IL-6(r=0.612),腹膜液IL-6(r=0.645),腹膜液miR-122(r=0.741)呈正相关。血清miR-122和miR-199 a的敏感性分别为96.6%和100.0%,对诊断子宫内膜异位症的特异性分别为94.4%和100%。结论子宫内膜异位症患者血清、腹膜液中的miR-122和miR-199a明显升高,表明这些miRNA可作为诊断子宫内膜异位症的生物标志物。 Objective To evaluate the value of serum miR-122 and miR-199a as reliable non-invasive biomarkers for the diagnosis of endometriosis.Methods Total of 80 patients with pelvic endometriosis was collected in January 2016 to January 2018 in our hospital and 70 women who underwent laparoscopy for pelvic pain but were not diagnosed with endometriosis for perspective study.Blood and peritoneal fluid samples were collected and the expression levels of interleukin-6(IL-6),miR-122,and miR-199a were measured by enzyme-linked immunosorbent assay.Results The levels of IL-6,miR-122 and miR-199a in serum and peritoneal fluid of patients with endometriosis were significantly higher than those in the control group(P<0.001).Serum miR-122 expression was correlated with serum IL-6(r=0.591),serum miR-199a(r=0.746),peritoneal fluid (IL-=0.611),peritoneal fluid miR-122(r=0.956),and peritoneal fluid miR-There was a positive correlation between 199a(r=0.678).At the same time,serum miR-199a expression was positively correlated with serum IL-6(r=0.612)IL-6(r=0.645)and peritoneal fluid miR-122(r=0.741).The sensitivities of serum miR-122 and miR-199 were 96.6%and 100.0%respectively.The specificities of endometriosis were 94.4%and 100%,respectively.Conclusion The serum levels of miR-122 and miR-199a in peritoneal fluid were significantly higher in patients with endometriosis,indicating that these miRNAs can be used as biomarkers for the diagnosis of endometriosis.

作者王悦菲蔡蓉何琳胡涛 Wang Yuefei;Cai Rong;He Lin;Hu Tao(Department of Gynecology,Wuxi Second People′s Hospital Affiliated to Nanjing Medical University,Wuxi 214000,Jiangsu,China;Department of Gynecology,Nanjing Maternal and Child Health Hospital,Nanjing 210000,Jiangsu,China.)

机构地区南京医科大学附属无锡第二人民医院妇科南京市妇幼保健院妇科

出处《贵州医药》 CAS 2020年第5期678-681,共4页 Guizhou Medical Journal

基金 2017年江苏省妇幼健康科研项目(F201758)。

关键词子宫内膜异位症 MIR-122 miR-199 a Endometriosis miR-122 miR-199a

分类号 R34 [医药卫生—基础医学] R711 [医药卫生—妇产科学]

引文网络
相关文献

参考文献1

1王洋,马彩虹,乔杰.子宫内膜异位症不孕患者着床窗口期内膜组织中微小RNA的差异表达及其意义[J].中华妇产科杂志,2016,51(6):436-441. 被引量：14

二级参考文献12

1Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets[J]. Cell, 2005, 120(1):15-20.
2Altmae S, Martinez-Conejero JA, Esteban FJ, et al. MicroRNAs miR-30b, miR-30d, and miR-494 regulate human endometrial receptivity[J]. Reprod Sci, 2013, 20(3):308-317.
3Livak K J, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Deha Delta C (T)) Method[J]. Methods, 2001, 25 (4) :402-408.
4Pan Q, Luo X, Toloubeydokhti T, et al. The expression profile of micro-RNA in endometrium and endometriosis and the influence of ovarian steroids on their expression[J]. Mol Hum Reprod, 2007, 13(11):797-806.
5Hou J, Wang P, Lin L, et al. MicroRNA-146a feedback inhibits RIG-I-dependent Type I IFN production in macrophages by targeting TRAF6, IRAK1, and IRAK2[J]. J hnmunol, 2009, 183(3):2150-2158.
6Taganov KD, Boldin MP, Chang K J, et al. NF-kappaB- dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses[J]. Proc Natl Acad Sci U S A, 2006, 103(33):12481-12486.
7Zhao JL, Rao DS, Boldin MP, et al. NF-kappaB dysregulation in microRNA-146a-deficient mice drives the development of myeloid malignancies[J]. Proc Natl Acad Sci U S A, 2011, 108(22):9184-9189.
8Labbaye C, Spinello I, Quaranta MT, et al. A three-step pathway comprising PLZF/miR- 146a/CXCR4 controls megakaryopoiesis [J]. Nat Cell Biol, 2008, 10(7):788-801.
9Starczynowski DT, Kuchenbauer F, Wegrzyn J, et al. MicroRNA-146a disrupts hematopoietic differentiation and survival[J]. Exp Hematol, 2011, 39(2):167-178.e4.
10Jazdzewski K, Murray EL, Franssila K, et al. Common SNP in pre-miR- 146a decreases mature miR expression and predisposes to papillary thyroid carcinoma[J]. Proc Natl Acad Sci U S A, 2008, 105(20):7269-7274.