云岭牛无角性状的分子鉴定与应用

Molecular Identification and Application of Polled Trait in Yunling Cattle

[目的]对云岭牛的无角性状进行分子鉴定,以加快云岭牛无角品系的培育速度。[方法]采用PCR扩增与琼脂糖电泳分型的方法。[结果]在263头云岭牛中,221头牛的基因型与其无角与有角性状完全一致,分子鉴定成功率为83.03%,还有42头云岭牛(16.97%)不能正确鉴定其无角与有角性状。[结论]分子鉴定技术可以有效鉴定云岭牛的无角与有角性状,将缩短云岭牛的无角品系选育进程。

[Objective]It will speed up the breeding of polled strain in Yunling cattle through identifying the polled trait of Yunling cattle using molecular genetics technology.[Method]PCR amplification and agarose gel electrophores methods were used.[Result]Among 263 Yunling samples,the genotypes of 221 Yunling samples were completely consistent with the polled and horned traits,the molecular identification rate was 83.03%.However,the polled and horned traits of 42 Yunling samples(16.97%)cannot be identified correctly.[Conclusion]The molecular identification technology is effective to identify the polled and horned traits of Yunling cattle,which will shorten the breeding program of the polled line in Yunling cattle.