摘要
采用超声波辅助酶法提取百香果籽中的可溶性膳食纤维(SDF),以期缩短提取时间并提高SDF得率。通过单因素探讨纤维素酶浓度、超声波功率、提取温度、料液比和水浴时间对百香果籽SDF得率影响,并在此基础上设计正交试验优化提取工艺条件。结果表明:超声波辅助酶法提取百香果籽SDF最佳工艺条件为纤维素酶浓度1.5%、超声波功率120 W、料液比(g/mL)1∶15、提取温度55℃、水浴时间160 min,以该工艺条件提取百香果籽SDF,提取率可达5.12%。
Ultrasound-assisted enzymatic extraction of soluble dietary fiber(SDF) from passion fruit seeds was carried out in order to shorten the extraction time and increase the yield of SDF. The effects of cellulase concentration, ultrasonic power, extraction temperature, solid-liquid ratio and water bath time on the SDF yield of passion fruit were studied by single factor analysis. And the orthogonal experiment was designed to optimize the extraction conditions. The results showed that the optimum conditions for the extraction of SDF from passion fruit by ultrasoundassisted enzymatic method were cellulase concentration 1.5%, ultrasonic power 120 W, solid-liquid ratio(g/mL) 1 ∶ 15, extraction temperature 55 ℃, and water bath time 160 min. The SDF extraction rate was determined to be 5.12%.
作者
杨翠凤
滕峥
吴观英
廖世孔
黄坤林
YANG Cui-feng;TENG Zheng;WU Guan-ying;LIAO Shi-kong;HUANG Kun-lin(Agricultural and Food Engineering College,Baise College,Baise 533000,PRC)
基金
硕士学位授予单位立项建设项目(091006001)
广西本科高校特色专业及实验实训教学基地(中心)建设项目(303011801)。
关键词
百香果籽
可溶性膳食纤维
超声波辅助
酶法提取
Passion fruit seeds
Soluble dietary fiber
Ultrasound-assisted
Enzymatic extraction