海洋真菌赤褐炭团菌FS521的次级代谢产物研究

Cytotoxic Secondary Metabolites from a Sea-Derived Fungal Strain of Hypoxylon rubiginosum FS521

从海洋真菌赤褐炭团菌(Hypoxylon rubiginosum)FS521中分离纯化得到17个聚酮类化合物,分别鉴定为4-乙酰氧基-8-甲氧基-3,4-二氢萘-1(2H)-酮(1)、4,8-二甲氧基-1-萘酚(2)、1'-羟基-4',8,8'-三甲氧基[2,2']联萘-1,4-二酮(3)、3,6-dimethyl-atromentin(4)、4-羟基-8-甲氧基-3,4-二氢萘-1(2H)-酮(5)、regiolone(6)、methylsclerone(7)、5-methylmellein(8)、3,5-二甲基-8-甲氧基-3,4-二羟基异香豆素(9)、9-甲氧基萘(10)、1,8-二甲氧基萘(11)、8-甲氧基-1-萘醇(12)、7-甲氧基-3-甲基异苯并呋喃(13)、3-甲基-4-羟基苯乙酸(14)、nodulisporipyrone A(15)、nodulisporipyrone B(16)、3,4-二甲氧基-乙酰苯乙醇(17).其中化合物1为新化合物,化合物2和3为新天然产物,新化合物的结构通过HRESIMS,1D NMR和2D NMR等光谱技术确定.此外,对分离所得的化合物1~4进行了体外细胞毒活性测试,结果表明化合物3对SF-268、MCF-7、HepG-2、A549有较强的细胞毒活性,其IC50值分别为1.85、3.21、2.53、5.09 mmol·L-1.

One new compound,named hypoxone A(1),together with two new natural products 4,8-dimethoxy-1-naphthol(2)and 1'-hydroxy-4',8,8'-trimethoxy[2,2']binaphthalenyl-1,4-dione(3),and fourteen known derivatives 4~17 were isolated from the broth extract of the marine fungus Hypoxylon rubiginosum FS521,which was isolated from a deep-sea sediment sample.Their structures were elucidated by interpretation of spectroscopic analysis and comparison with previously published data for the known ones.Moreover,compounds 1~4 were evaluated for their in vitro cytotoxic activities against SF-268,MCF-7,HepG-2,and A549 tumor cell lines,and compound 3 exhibited potent cytotoxic activity with IC50 values of 1.85,3.21,2.53,and 5.09 mmol·L-1,respectively.