过敏性哮喘患儿外周血单个核细胞mir-29c与Th1/ Th2细胞平衡的相关性分析

Correlation between mir-29c and Thl/Th2 cell balance in peripheral blood mononuclear cells of children with allergic asthma

目的分析过敏性哮喘(AA)患儿外周血单核细胞微小RNA 29c(mir-29c)表达及与CD4+辅助性T细胞1/CD4+辅助性T细胞2(Th1/Th2)平衡的相关性。方法将2015年10月-2018年12月本院收治的94例AA患儿分为稳定组32例,轻度急性发作组27例,中度急性发作组35例;另取健康体检儿童40例为对照组。采集所有受试者儿童外周血,分离血清和单个核细胞(PBMC),检测mir-29c、Th1/Th2、IFN-γ、IL-4、T盒子转录因子21(T-bet)、GATA结合蛋白3(GATA3)蛋白水平。结果与对照组比较,稳定组、轻、中度急性发作组AA患儿PBMC中mir-29c水平、Th2亚群比例、GATA3蛋白水平及血清IL-4水平依次升高,PBMC中Th1亚群比例、Th1/Th2、T-bet蛋白水平、T-bet/GATA3及血清IFN-γ水平、IFN-γ/IL-4依次降低,两两比较差异均有统计学意义(P<0.05)。AA患儿外周血mir-29c水平与Th1/Th2、IFN-γ/IL-4、T-bet/GATA3均呈负相关(r=-0.823、-0.789、-0.804,P<0.05)。结论 mir-29c在AA患儿外周血单个核细胞中上调表达,可能通过Th1/Th2平衡影响AA发生。

Objective To analyze the expression of microRNA 29 c(mir-29 c) in peripheral blood mononuclear cells of children with allergic asthma(AA) and its correlation with the balance of CD4+helper T cell 1/CD4+helper T cell 2(Th1/Th2).Methods From October 2015 to December 2018,Totally 94 children with AA were divided into stable group(32 cases),mild acute attack group(27 cases) and moderate acute attack group(35 cases),and another 40 healthy children underwent physical examination were selected as the control group.Peripheral blood was collected from all subjects,serum and peripheral blood mononuclear cell(PBMC) were separated,the levels of mir-29 c,Th1/Th2,IFN-gamma,IL-4,T-box transcription factor(T-bet) and GATA binding protein 3(GATA3) were detected.Results Compared with the control group,the levels of mir-29 c,Th2 subgroup,GATA3 protein in PBMC and the level of IL-4 in serum of children with AA in stable group,mild and moderate acute attack group increased in turn,while the percentage of Th1 subgroup,Th1/Th2,T-bet protein level,T-bet/GATA3,serum IFN-γ level and IFN-γ/IL-4 in PBMC decreased in turn,and there was significant difference between any two groups(P<0.05).The levels of mir-29 c in peripheral blood of children with AA were negatively correlated with Th1/Th2,IFN-γ/IL-4 and T-bet/GATA3(r=-0.823,-0.789,-0.804,P<0.05).Conclusion Mir-29 c is up-regulated in peripheral blood mononuclear cells of children with AA and may affect the occurrence of AA through Th1/Th2 balance.