北方旱地红小豆DREB2A基因克隆及表达分析

Cloning and Expression Analysis of DREB2A Gene in Red Bean in Northern Arid Region of China

脱水响应元件结合蛋白(DREB)转录因子在植物干旱和热胁迫应激调控中具有重要功能。为提高红小豆在北方干旱缺水条件下的产量,本研究以拟南芥DREB2A基因序列为依据,从红小豆基因组数据库中鉴定出了1个DREB2A的同源基因(Vigna angularis,登录号为XP_017429189.1)。经基因表达分析,结果表明:此基因为拟南芥DREB2A的直系同源基因,编码的蛋白不具有信号肽的作用,二级结构主要以无规则卷曲为主,而且含有多种逆境性转录因子。通过物种间系统进化树分析发现,该基因与红小豆DREB2A亲缘关系近的植物是沙冬青。荧光定量PCR技术分析结果表明,在红小豆非生物胁迫的调控中红小豆DREB2A也起到重要作用。本研究为阐释红小豆抗逆分子机制提供理论基础。

Dehydration responsive element binding protein(DREB)transcription factor plays an important role in plant drought and heat stress regulation.In order to improve the yield of red bean under drought and water deficit conditions in the north,a DREB2A homologous gene(XP_017429189.1)was identified from the genome database of red bean based on the DREB2A gene sequence of Arabidopsis thaliana.The results of gene expression analysis showed that the gene is a direct homologous gene of DREB2A in Arabidopsis thaliana.The encoded protein did not have signal peptide.The secondary structure is mainly random coil and contained many stress transcription factors.Phylogenetic tree analysis showed that the DREB2A gene of red bean closely related to Ammopiptanthus mongolicus(Maxim.)Cheng f..The results of quantitative fluorescence PCR showed that DREB2A also play an important role in the regulation of abiotic stress in red bean.This study provides a theoretical basis for elucidating the molecular mechanism of stress resistance of red bean.