辣椒转录因子CaNAC23基因的克隆与表达分析

Cloning and Expression Analysis of CaNAC23 Gene in Capsicum annuum L.

为分析NAC转录因子在辣椒发育及非生物胁迫中的功能,本研究以‘晋尖椒22’为试验材料,克隆获得CaNAC23基因全长,该基因全长1899 bp,编码632个氨基酸,预测分子量约为72.54 kD,等电点为6.26,无序化区域有7个。二级结构预测和亚细胞定位预测表明CaNAC23含有一个保守的NAC结构域,定位于细胞核。氨基酸序列比对和进化树分析表明CaNAC23和拟南芥AT3G03200(NAC045)同源性较高,进化上在同一分支。CaNAC23基因的组织表达特异性和非生物胁迫下的表达模式结果表明:CaNAC23在叶片中表达量最高,其次是根,暗示CaNAC23基因可能参与辣椒叶片或根发育相关。CaNAC23能够被干旱和盐胁迫诱导表达,推测其参与调控辣椒的非生物胁迫调控。本研究结果为研究NAC转录因子在辣椒发育及非生物胁迫应答中的功能提供了参考依据。

In order to analyze the function of NACs in pepper development and abiotic stress,the full length of CaNAC23 gene was obtained using'Jinjianjiao 22'as test material.The results showed that the total length of the gene was 1899 bp,encoding 632 amino acids,the predicted molecular weight was about 72.54 kD,the isoelectric point was 6.26,and there were 7 disordered regions.Secondary structure prediction and subcellular localization prediction indicated that CaNAC23 contained a conserved NAC domain and located in the nucleus.Through amino acid sequence comparison and phylogenetic analysis of CaNAC23,it was proved that CaNAC23 was clustered with Arabidopsis AT3G03200(NAC045).The tissue expression specificity and abiotic stress expression pattern of CaNAC23 gene showed that CaNAC23 had the highest expression level in leaves,followed by roots,suggesting that CaNAC23 gene might be involved in the development of leaves or roots.CaNAC23 can be induced to express by drought and salt stress,indicating that it is involved in the regulation of abiotic stress in pepper.Therefore,these results providing a reference for the study of the function of NACs in pepper development and abiotic stress response.