摘要
为筛选出彩色马蹄莲不同品种和组织最佳内参基因,以彩色马蹄莲不同品种和不同组织为研究材料,运用实时荧光定量PCR技术(qRT-PCR)结合3个内参基因稳定性分析软件(geNorm,NormFinder和BestKeeper),对彩色马蹄莲6个候选内参基因(18S rRNA,ACT,EF1α,GAPDH,LEU和TUB)的表达稳定性进行了分析。通过geNorm软件计算出彩色马蹄莲不同品种和组织的最适内参基因数目均为3个;综合3个软件的分析结果,筛选出彩色马蹄莲不同品种中稳定性最好的3个基因为18S rRNA、LEU和GAPDH;不同组织中稳定性最佳的3个基因为ACT、EF1α和18S rRNA。本研究为彩色马蹄莲品种间叶片表型差异、种球发育、赤霉素敏感差异等机理研究提供参考依据。
In order to screen out the suitable reference genes for different varieties and tissues of colored calla lily(Zantedeschia hybrida),the different varieties and tissues of colored calla were used as experimental materials,the expression stability of 6 candidate reference genes(18S rRNA,ACT,EF1α,GAPDH,LEU and TUB)in colored calla lily was analyzed by real-time fluorescent quantitative PCR combined with three softwares(geNorm,NormFinder and BestKeeper).According to geNorm software calculation results,the optimal number of reference genes for different varieties and tissues of colored calla lily was 3.According to the analysis results of three softwares,the three most stable genes in different varieties of colored calla were lily 18S rRNA,LEU and GAPDH,and in different tissues were ACT,EF1αand 18S rRNA.This study provides a reference for the mechanism study of leaf phenotypic differences,bulb development,and gibberellin sensitivity differences among different varieties of colored calla lily.
作者
周琳
张永春
蔡友铭
赵冰雪
付椿淇
杨柳燕
Zhou Lin;Zhang Yongchun;Cai Youming;Zhao Bingxue;Fu Chunqi;Yang Liuyan(Shanghai Key Laboratory of Protected Horticultural Technology,Forestry and Pomology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201403;School of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai,201418;Agricultural College,Anshun University,Anshun,561000)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第12期3971-3979,共9页
Molecular Plant Breeding
基金
上海市现代农业产业技术体系(沪农科产字(2019)第8号)
上海市科技兴农成果转化项目(沪农科转字(2016)第2-2号)共同资助。
