摘要
呼吸道感染性疾病严重威胁儿童健康,早期明确病原可为疾病的诊断和治疗奠定基础。宏基因组测序是一种不依赖于培养,直接对临床样品进行测序,并给出全部微生物序列信息的技术。宏基因组测序从病原微生物的检测、微生物组学、微生物与宿主基因相互作用等方面为儿童呼吸道感染性疾病病原微生物的研究提供了十分重要的研究方法。现从宏基因组测序流程、在儿童呼吸道感染性疾病中的应用及国内外研究进展等方面进行阐述。
Respiratory infectious diseases are a serious threat to children health.The early identification of the pathogen will lay the foundation for the diagnosis and treatment of the diseases.Metagenomics is a technique which directly sequences clinical samples without culture and reveals the information about all microbial sequences.It plays a very important part in the detection of pathogenic microorganisms,microbiology and interaction between microorganisms and host genes for the study of pathogenic organisms in children′s respiratory infectious diseases.In this article,the sequencing processes of metagenomics,its application in children′s respiratory infectious diseases and research progress at home and abroad were reviewed.
作者
徐放
申阿东
Xu Fang;Shen Adong(Beijing Key Laboratory of Pediatric Respiratory Infection Diseases,Key Laboratory of Major Disease in Children,Ministry of Education,National Clinical Research Center for Respiratory Diseases,National Key Discipline of Pediatrics(Capital Medical University),Beijing Pediatric Research Institute,Beijing Children′s Hospital,Capital Medical University,National Center for Children′s Health,Beijing 100045,China)
出处
《中华实用儿科临床杂志》
CSCD
北大核心
2020年第10期783-786,共4页
Chinese Journal of Applied Clinical Pediatrics
基金
国家自然科学基金面上项目(81571950)
北京市医院管理中心儿科学科协同发展中心专项(XTZD20180503)。
关键词
呼吸道感染性疾病
高通量测序
宏基因组测序
Respiratory infection diseases
High throughput sequencing
Metagenomics
