发生盆腔淋巴结转移的宫颈鳞癌组织微小RNA表达谱分析

Analysis of MicroRNA Expression Profile of Cervical Squamous Cell Carcinoma with Pelvic Lymph Node Metastasis

目的:探讨发生盆腔淋巴结转移的宫颈鳞癌组织微小RNA(miRNA)表达谱特征。方法:采用miRNA 4.0芯片检测宫颈鳞癌淋巴结转移(n=8)和未转移患者(n=16)组织中表达的miRNAs。结果:两组间有5个miRNAs差异表达具有统计学意义(P<0.05),且均表现为上调,其中miRNA-8073和miRNA-5585-3p在以往宫颈癌相关miRNA研究中未见报道。结论:本研究结果显示miRNA-8073和miRNA-5585-3p与宫颈鳞癌盆腔淋巴结的转移有关,可能成为宫颈癌淋巴结转移的标志物或治疗靶点。

Objective To investigate the microRNA expression profile characteristics of cervical squamous cell carcinoma with pelvic lymph node metastasis.Methods Expression differences of miRNAs in lymph node metastasis tissue(n=8)andnon-metastasis tissue(n=16)from patients with Squamous cell carcinoma of cervix(SCC)were analyzed by miRNA 4.0 array.Results Expression of five miRNAs increased in lymph node metastasis tissue compared with non-metastasis tissue(P<0.05),in which the miRNA-8073 and miRNA-5585-3phave not been reported so far.Conclusions The Results of this study show that the miRNA-8073 and miRNA-5585-3p were related with the pelvic lymph node metastasis of SCC.