利用RAPD标记分析主产地草果遗传多样性

Genetic Diversity Analysis of Amomum tsao-ko in Main Producing Area Using RAPD Markers

以云南省红河州4个居群的48份草果为试材,采用RAPD分子标记技术,研究草果遗传多样性,以期为草果资源的保护及利用提供参考依据。结果表明:11条RAPD引物共扩增出139个条带,多态性条带比率(PPB)为98.56%。在群体水平上,金平居群的遗传多样性最高(PPB=82.01%),其次是绿春居群(PPB=66.91%),屏边居群(57.55%)和元阳居群(55.40%)多样性水平相对较低。草果总遗传多样性的87.67%(Hs=0.197)来自于居群内部,居群间遗传变异只占12.33%(Gst=0.123),AMOVA分析进一步证明草果的遗传变异主要存在于居群内部。遗传一致度分析表明不同居群间的遗传一致度较高,相似性系数在0.9314~0.9717,表明草果居群间遗传变异较小。

The genetic diversity of 48 Amomum tsao-ko from four populations in Honghe Prefecture of Yunnan Province was analyzed by random amplified polymorphic DNA(RAPD)molecular marker technique.This study could provide an important theoretical basis for the protection and utilization of A.tsao-ko resources.The results showed that a total of 139bands were amplified by 11 RAPD primers,and the ratio of polymorphic bands(PPB)was 98.56%.At the population level,Jinping population had the highest genetic diversity(PPB=82.01%),followed by Lvchun population(PPB=66.91%),Pingbian population(57.55%)and Yuanyang population(55.40%).87.67%(Hs=0.197)of the total genetic diversity of A.tsao-ko came from within populations,and the genetic variation among populations accounted for only 12.33%(Gst=0.123),analysis of molecular variance(AMOVA)further proved that the genetic variation of A.tsao-ko mainly existed within populations.Four A.tsao-ko populations shared high levels of genetic identity(0.9314-0.9717),and the genetic variation among the populations was small.