超低温肝脏保存技术的实验研究进展

Advances in supercooling liver preservation

肝移植是目前治疗终末期肝病唯一有效的方法。移植前供肝的保存至关重要,传统的静态冷藏和机械灌注均受保存时间的限制,以至于供肝的分配空间有限,不可避免地造成部分供肝的废弃。在保证供肝质量的同时,寻找更长的有效保存供肝的技术,是所有肝移植科医师共同努力的方向。最近报道的一种超低温肝脏保存技术可以增加肝脏保存时间,利用冷冻保护剂将肝脏在-6℃下保存3~4 d,之后经过亚常温机械灌注复苏供肝后进行肝移植。该技术在供肝保存方面取得了革命性突破,可能为供肝保存研究开拓出新的领域。但超低温肝脏保存技术目前仅处于实验阶段,将其应用于临床实践还有待进一步深入探究。

Liver transplantation is currently the only effective treatment for end-stage liver disease.The preservation of donor liver before transplantation is important.But both traditional static cold storage and machine perfusion are limited by the preservation time,so that the allotment space of donor liver is limited,which inevitably leads to the abandonment of part of donor liver.At present,to find a preservation technology that not only guarantees the quality of donor liver but also has a longer effective preservation time is the direction of joint efforts of all clinicians.Supercooling liver preservation(SLP)to find a preservation technology that not only guarantees the quality of donor liver but also has a longer effective preservation time is the direction of joint efforts of all clinicians.SLP,a new method based on using cryoprotectants to keep donor liver under-6℃and recovering the graft with subnormothermic machine perfusion that enables long-term transplantation survival following 4 days of liver preservation,made a revolutionary breakthrough in the field of liver preservation,carved out a new field for the research of liver preservation.This article reviews the latest experimental research progress of SLP in the field of liver transplantation.