摘要
Background:Fourier transform infrared(FTIR)spectroscopy technique has not been used as a diagnostic tool for diabetes in clinical practice.This study was linked to structural changes in hemoglobin(Hb)in type 2 diabetes patients at higher levels of HbA 1C using FTIR spectroscopy.Methods::Fifty-three diabetic patients from the Bahawal Victoria Hospital,Bahawalpur,Pakistan were categorized as group A(6%<HbA 1C<7%;n=25)and group B(HbA 1C≥9%;n=28).Another group(group N)of twenty blood samples was taken from healthy people from the Islamia University Bahawalpur,Pakistan.Data from all groups were collected from January 1,2018 to March 31,2019.The structure of Hb was studied by FTIR spectroscopy and impact of glucose on the fine structure of HbA 1C was estimated.Results::Hb secondary structure erythrocyte parameters were altered by changing glucose concentrations.From FTIR spectra of all three groups it was found that Hb structure was slightly altered in group A,but significantly changed in group B(P<0.05).There was an increase inβ-sheet structure and a reduction inα-helix structure at elevated levels of HbA 1C(group B)in type 2 diabetes.Conclusion::We suggest that higher level of glycation reflected by increased HbA 1C might be a contributing factor to structural changes in Hb in type 2 diabetic patients.FTIR spectroscopy can be a novel technique to find pathogenesis in type 2 diabetes.
