蚕豆叶片气孔对生长素和脱落酸的反应

Stomatal Responses of Vicia faba L. to IAA and ABA

研究了蚕豆叶片和离体表皮条上气孔对外源生长素和脱落酸的反应及这2种激素在调节气孔运动中的关系。生长素能诱导关闭的气孔在黑暗条件下开放，在0．01～10μmol·L-1浓度范围内，随生长素浓度增加，气孔孔径加大，但在更高浓度下，诱导作用减小；叶片上的气孔比离体表皮条上的气孔对生长素的浓度变化反应更敏感。在0．0f～10μmol·L-1浓度范围内，脱落酸能有效地诱导气孔关闭，随脱落酸浓度增加而气孔孔径减小；在较低浓度范围，离体表皮上的气孔反应较灵敏，但在较高浓度时，叶片上的气孔关闭更明显。抑制被光诱导的气孔开放的作用随脱落酸浓度增加而加强，在叶片上的气孔和在离体表皮上的气孔对脱落酸的反应有类似趋势，但叶片上气孔孔径变化的幅度更大。生长素对脱落酸诱导的气孔关闭表现出一定程度的拮抗作用，与其不同浓度组合有关。

Effects of external ABA and IAA on the stomatal behavior of Vicia faba L. were tested with leaf tissues and isolated epidermal peels. Tissues were incubated in solutions containing different concentration IAA or/and ABA in the light or dark. In the range of 0.01～10 μmol· L-1 concentration IAA induced stomatal opening in the dark, stomatal apertures reached maximum at 10 μmol·L-1. Stomata in leaf tissues were apparently more sensitive to IAA than isolated epidermal peels. In the range of 0.01～10 μmol·L-1ABA induced stomatal closure and inhibited stomatal open in the light. In terms of inducing stomotal closure,stomata in isolated epidermis were more sensitive to the low concentration (0. 01～0. 1 μmol ·L-1) of ABA, but stomatal aperture reduced rapidly in leaf tissues at the 1～10 μmol·L-1ABA concentration. For inhibiting light induced stomotal opening, effects increased with rising ABA concentration, but the changes of stomatal aperture were more notable in leaf tissues. Interactions between IAA and ABA on closing regulation of stomata were also studied,and results showed that IAA and ABA are antagonistic each other in their effects on stomatal movement.