刺芒柄花素对Ⅱ型糖尿病大鼠骨质疏松的缓解作用

Alleviation Effect of Formononetin on Osteoporosis in Type 2 Diabetes Mellitus Complicated with Diabetic Bone Disease

目的探究刺芒柄花素对Ⅱ型糖尿病并发糖尿病骨病大鼠骨质疏松的缓解作用。方法使用链脲佐菌素(STZ)+高脂饲料联合的方式建立Ⅱ型糖尿病大鼠模型,分别以灌胃方式给予糖尿病模型大鼠高、中、低质量浓度(100 mg/kg、50 mg/kg和20 mg/kg)的刺芒柄花素2周后,检测各组大鼠股骨骨密度、血清睾酮(T)和雌二醇(E2)水平,以及大鼠股骨骨钙素(OC)、肾脏CYP24A1 mRNA表达水平。结果与正常组相比,Ⅱ型糖尿病组的骨密度、血清T和E2水平、股骨OC和肾脏CYP24A1 mRNA表达均下降;而刺芒柄花素能逆转上述现象,并且高浓度组的效果最好。结论刺芒柄花素对Ⅱ型糖尿病并发糖尿病骨病大鼠的骨质疏松具有缓解作用。

Objective To investigate whether the formononetin has a mitigating effect on osteoporosis in type 2 diabetic rats with diabetic bone disease.Methods A model of type 2 diabetes mellitus was established by combining Streptozotocin(STZ)+high-fat diet.High,medium and low dosages(100 mg/kg,50 mg/kg,20 mg/kg)of formononetin were administered by intragastric administration for 2 weeks,followed by detection of rats in each group.The bone mineral density,serum testosterone(T)and estradiol(E2)levels,as well as the mRNA expressions of osteocalcin(OC)in femur and CYP24A1 in kidney of rats were measured.Results Compared with the control group,the bone mineral density,serum T and E2 levels,femoral OC,and renal CYP24A1 mRNA expression decreased in the type 2 diabetes group,while the formononetin could reverse the above phenomenon,and the high dosage group had the most positive effect.Conclusion Formononetin can alleviate osteoporosis in type 2 diabetic rats with diabetic bone disease.