大骨节病患者软骨组织中FAS/DR4死亡受体膜蛋白高表达

High expression of death receptor membrane proteins in the cartilage of Kashin-Beck disease

目的研究大骨节病关节软骨组织中凋亡相关的死亡受体4/5(DR4/5)及FAS的表达变化。方法按照大骨节病诊断标准收集大骨节病患者关节软骨标本(KBD组),以原发性骨关节病关节软骨(OA组)作为病例对照,以正常关节软骨(NC组)作为健康对照,制备石蜡切片,进行免疫组织化学染色标记法检测凋亡途径中重要死亡受体DR4/5及FAS的表达,光学显微镜下计数并计算软骨细胞阳性表达率,统计分析组间差异。结果在软骨组织表层,KBD组、OA组的FAS阳性细胞率明显高于NC组,KBD组的DR4及DR5与NC组差异均无统计学意义;在中层,KBD组和OA组的FAS、DR4阳性细胞率均明显高于NC组,KBD组的DR5与NC组无差异;在深层,KBD组的FAS阳性细胞率明显高于NC组,KBD组和OA组的DR4阳性细胞率均明显高于NC组,KBD组的DR5与NC组无差异。结论FAS/DR4主要表达于深层及中层,提示FAS/DR4介导的细胞死亡可能参与了深层软骨坏死这一特征性的病理变化。

Objective To study the changes of apoptosis-related death receptor 4/5(DR4/5)and FAS expressions in the cartilage from Kashin-Beck disease(KBD).Methods Cartilage specimens of KBD were collected according to the diagnostic criteria of KBD,primary osteoarthrosis(OA)was used as a case control and normal articular cartilage(NC)as a healthy control.The important molecule of DR signaling was detected by immunohistochemical staining.The results were counted under light microscope and the positive rate was calculated.The expression differences of the three groups of cartilage tissues were statistically analyzed.Results The FAS-positive cell rate in the superficial layer of KBD was significantly higher than that in the NC group,and it was higher in OA group than in NC group.The expressions of DR4 and DR5 in KBD group were not significant compared with those in NC group.In the middle layer,both FAS-and DR4-positive cell rates were higher in KBD and the OA cartilage than in the NC control.DR5 expression did not differ between NC group and KBD group.In the deep layer,the FAS positive cell rate in KBD was significantly higher than that in NC,and the DR4 positive cell rate in KBD and OA groups was significantly higher than that in NC group.DR5 did not differ between NC andKBD groups.Conclusion The expression of FAS/DR4 was observed in the deep and middle layers of KBD joints.It suggests that FAS/DR4-mediated cell death may be involved in the characteristic pathological changes of deep cartilage necrosis.