不同日龄鹅胚成纤维细胞的分离培养及活力研究

Isolation,culture and viability of embryo fibroblasts from goose at different ages

选择发育良好的9、11、13和15胚龄的马岗鹅鹅胚各3枚,分离鹅胚成纤维细胞并培养,于24、48、72、96、120和144 h分别进行细胞计数绘制不同胚龄成纤维细胞的生长曲线、检测细胞活力及细胞增殖率.结果显示,9、11日龄鹅胚成纤维细胞状态更好,死细胞和杂细胞较少;11日龄鹅胚成纤维细胞的细胞生长曲线最理想,活细胞比例为4个日龄中最高;9、11和13日龄鹅胚成纤维细胞的增殖率均保持在较高的水平.11日龄鹅胚更适合用于分离培养成纤维细胞.

Fibroblasts isolated from three well-developed 9,11,13 and 15 embryonic age embryos of Magang geese were cultured for 24,48,72,96,120 and 144 h,and the cell was counted,different age of embryo fibroblast growth curve was drawn,cell viability and cell proliferation rate was detected.The results showed that 9 and 11-day-old goose embryo fibroblasts were in better condition with fewer dead cells and hetero cells;the growth curve of 11-day-old goose embryo fibroblast cells was the best with the highest proportion of living cells;the proliferation rate of 9,11 and 13-day-old geese embryo fibroblasts maintained higher levels.Eleven-days-old geese embryo was more suitable for isolation and culture of fibroblasts.