生血宝合剂浓缩液质量快速分析方法研究

Study on rapid quality analysis method of Shengxuebao Mixture

该研究基于紫外-可见光谱、近红外光谱及多变量数据处理技术,提出了一种生血宝合剂浓缩液的质量快速分析方法,用于同时预测生血宝合剂浓缩液中芍药内酯苷、芍药苷、二苯乙烯苷、特女贞苷、旱莲苷D、大黄素、毛蕊异黄酮苷和黄芪甲苷8种有效成分的含量。以高效液相色谱-紫外-质谱联用分析法作为有效成分含量测定的参照方法,使用偏最小二乘回归法建立生血宝合剂浓缩液紫外-可见光谱或近红外光谱与8种成分含量之间的多元校正模型,最后以24批测试集样本对模型性能进行评价。结果表明:生血宝合剂浓缩液中8个有效成分的紫外-可见光谱校正模型和近红外光谱校正模型的决定系数均高于0.9,紫外-可见光谱校正模型外部验证的预测决定系数均高于0.83,近红外光谱校正模型外部验证的预测决定系数均高于0.79,该方法准确、稳健、快速,适用于生血宝合剂浓缩液质量的快速评价。

A rapid analysis method based on ultraviolet-visual(UV-Vis)spectroscopy,near infrared(NIR)spectroscopy and multivariable data analysis was established for quality evaluation of Shengxuebao Mixture.The contents of eight active ingredients of Shengxuebao Mixture including albiflorin,paeoniflorin,2,3,5,4'-tetra-hydroxy-stilbene-2-O-β-D-glucopyranoside,specnuezhenide,ecliptasaponin D,emodin,calycosin-7-glucoside and astragalosideⅣwere simultaneously detected by using this method.HPLC-UVMS was used as a reference method for determining the contents of these ingredients.Partial least squares(PLS)analysis was implemented as a linear method for multivariate models calibrated between UV spectrum/NIR spectrum and contents of 8 ingredients.Finally,the performance of the model was evaluated by 24 batches of test samples.The results showed that both UV-Vis and NIR models gave a good calibration ability with an R2 value above 0.9,and the prediction ability was also satisfactory,with an R2 value higher than 0.83 for UV-Vis model and higher than 0.79 for NIR model.The overall results demonstrate that the established method is accurate,robust and fast,therefore,it can be used for rapid quality evaluation of Shengxuebao Mixture.