红参和红景天配伍前后主要成分及抗疲劳活性的变化

Changes in Effective Compounds and Anti-fatigue Activity Before and After Compatibility of Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma

目的:初步阐释红参和红景天在化学和药效学层面的配伍机制,为临床应用提供理论依据。方法:利用高分离度快速液相色谱与四极杆-飞行时间质谱联用(RRLC-Q-TOF-MS)分析了红参和红景天配伍前后化学成分的变化,并通过负重游泳实验及血清尿素、血乳酸、肝糖原的测定考察了红参和红景天配伍前后的抗疲劳活性变化。结果:在红参和红景天配伍合提液中共鉴别了51种化学成分,丙二酰基人参皂苷(mRg1,mRb1,mRb2,mRb3和mRd等)含量明显降低,人参皂苷(Rb1,Rb2,Rb3,Rd,F2和Rg3等)含量明显升高。药效实验显示,与空白组比较,合提液组和合并液组小鼠竭力游泳时间均明显延长(P<0.01);两组小鼠的血清尿素氮(P<0.05,P<0.01)和乳酸(P<0.05,P<0.01)水平明显降低,而肝糖原水平明显升高(P<0.05,P<0.01)。红参和红景天配伍合提液抗疲劳作用较红参和红景天单煎合并液明显增强。结论:该研究在化学成分变化方面揭示了红参和红景天配伍过程中的增效机制,为临床应用和产品开发提供了理论参考。

Objective:To preliminarily interpret the compatibility of Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma in chemical and pharmacodynamic levels,and provide theoretical basis for its clinical application. Method: Rapid resolution liquid chromatography coupled with quadrupoletime-of-flight tandem mass spectrometry(RRLC-Q-TOF-MS)was applied to identify and analyze the changes in chemical components of the Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma before and after compatibility. The anti-fatigue activity before and after compatibility of Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma was detected by weight-loading swimming experiment and determination of levels of serum urea,blood lactic acid and hepatic glycogen. Result:A total of 51 compounds were identified in mixture decoction of Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma. Malonyl ginsenoside mRg1,mRb1,mRb2,mRb3 and mRd contents were significantly decreased,while ginsenoside Rb1,Rb2,Rb3,Rd,F2 and Rg3 contents were significantly increased in the compatibility mixture.According to pharmacodynamics study,as compared with those in the blank control group,swimming time of mice was significantly prolonged in all other groups(P<0. 01),serum urea nitrogen(P<0. 05,P<0. 01) and lactic acid(P<0. 05,P<0. 001)levels of mice in the combined decoction and the single decoction groups were significantly lowered,while liver glycogen levels were significantly elevated(P<0. 05,P<0. 01). The antifatigue ability of the combined decoction of Ginseng Radix et Rhizoma Rubra and Rhodiolae Crenulate Radix et Rhizoma was higher than that of the single decoctions. Conclusion: In this article,the effect enhancing mechanism of compatibility of Ginseng Radix et Rhizoma Rubra with Rhodiolae Crenulate Radix et Rhizoma was revealed based on the chemical changes,providing theoretical reference for the clinical application and development of products.