马兰头总黄酮的大孔树脂纯化工艺优化

Optimization of purification process of total flavonoids from Kalimeris by macroporous resin

目的利用大孔树脂来纯化马兰头中粗黄酮,并确定纯化黄酮的最佳工艺。方法以黄酮回收率为指标,在单因素实验的基础上运用Box-Behnken响应面法(response surface methodology,RSM)设计三因素三水平实验以获得最佳纯化条件。结果 HPD-600大孔吸附树脂纯化马兰头粗提液的最佳工艺条件为:上样浓度0.93 mg/mL、上样pH为3.00、洗脱剂体积分数为84.17%、吸附速率1 BV/h,洗脱速率1 BV/h,此条件下马兰头总黄酮的质量分数由纯化前的4.11%提高到纯化后的50.80%。结论利用HPD-600型大孔树脂可以较好地纯化马兰头中的总黄酮。

Objective To purify crude flavonoids from Kalimeris by macroporous resin and determine the optimum purifying process for flavonoids.Methods Based on the single factor experiment,Box-Behnken (response surface methodology,RSM) was used to design a three-factor and three-level experiment to obtain the best purification conditions with flavone recovery rate as the index.Results The optimum conditions for purification of crude extract of Kalimeris with HPD-600 macroporous adsorption resin were as follows:loading concentration 0.93 mg/mL,loading pH 3.00,eluent volume fraction 84.17%,adsorption rate 1 BV/h,and elution rate 1 BV/h.Under these conditions,the mass fraction of total flavonoids in Kalimeris was increased from 4.11% before purification to 50.80% after purification.Conclusion HPD-600 macroporous resin can be used to purify the total flavonoids in Kalimeris.