响应面法和正交实验法优化天山岩黄芪多酚提取纯化工艺

Optimization of the extraction and purification process of polyphenols from Hedysarum semenovii Regel et Herder by response surface method and orthogonal experiment method

目的采用响应面法和正交实验法对天山岩黄芪中多酚的提取及分离纯化工艺进行优化。方法采用单因素和响应面法确定天山岩黄芪多酚提取最佳工艺;筛选最佳型号的大孔吸附树脂,利用正交实验法优化大孔吸附树脂对天山岩黄芪多酚纯化分离的工艺。结果天山岩黄芪多酚提取的最佳工艺参数为:提取所用乙醇浓度为60%(V/V),料液比为1:25(m/V),提取温度为60℃提取时间为1.5 h。采用此方法提取天山岩黄芪多酚的含量为0.3316 mg/g;对天山岩黄芪多酚纯化的最佳大孔吸附树脂型号为HPD 500(径高比为1:5),最佳工艺参数为:上样量0.3柱床体积(bed volume,BV,1 BV=90 mL),65%乙醇洗脱,上样浓度为9%,此方法纯化天山岩黄芪多酚,含量为40.94 mg/g。结论采用响应面法实验优化法提取天山岩黄芪多酚,工艺稳定可靠;HPD500型大孔吸附树脂对天山岩黄芪多酚类物质纯化效果良好,可为天山岩黄芪中多酚的制备工艺提供参考。

Objective To optimize the extraction and purification process of polyphenols from Hedysarum semenovii Regel et Herder by response surface method and orthogonal experiment method.Methods Single factor and response surface method were used to determine the best extraction process of polyphenols from Hedysarum semenovii Regel et Herder.The best type of macroporous adsorption resin was selected and orthogonal test was used to optimize the purification and separation process of polyphenols.Results The best extraction parameters were that the ethanol concentration was 60%(V/V),the solid-liquid ratio was 1:25(m/V),the extraction temperature was60℃ and extraction time was 1.5 h,under these conditions the polyphenol content was determined as 0.3316 mg/g.The best type of macroporous adsorption resin was HPD500(diameter height ratio 1:5),and the best process parameters were showed as follows:the sample amount was 0.3 bed volume(BV,1 BV=90 mL),the concentration of sample was 9%,and eluted with 65% ethanol,under these condition the polyphenol content increased to 40.94 mg/g.Conclusion The response surface optimization method is stable and reliable for the extraction of polyphenols from Hedysarum semenovii Regel et Herder,and HPD 500 macroporous adsorption resin has good effect on the purification,which can provide a reference for the preparation of polyphenols in Hedysarum semenovii Regel et Herder.