益生菌/草本提取物复方清咽作用的实验研究

Experimental study on throat-clearing effect of the combination of probiotics and herbal extract

目的探讨益生菌/草本提取物复方的清咽作用。方法大鼠灌胃给予益生菌和/或草本提取物45 d,依据2012修订的保健食品"清咽功能检验方法"进行大鼠棉球植入实验、大鼠足趾肿胀实验及小鼠耳肿胀实验。结果棉球植入实验中,益生菌高剂量、草本中剂量和高剂量、益生菌低剂量+草本中剂量、益生菌低剂量+草本高剂量、益生菌高剂量+低/中/高剂量草本剂量组,大鼠肉芽肿净量显著低于对照组(P<0.05),具有干预肉芽肿(慢性炎症)形成的作用;足趾肿胀实验中,致炎1 h后,草本高剂量组大鼠足趾肿胀率显著降低(P<0.05),致炎1、2、6h后,益生菌高剂量+草本高剂量组足趾肿胀率均显著低于对照组(P<0.05)且低于益生菌高剂量组和草本高剂量组,提示益生菌草本高剂量复方干预致炎剂诱发的足趾肿胀作用效果优于单独给予高剂量益生菌或高剂量草本;小鼠耳廓肿胀实验中,各组耳廓肿胀值及肿胀率均无显著变化。结论高剂量草本和益生菌草本高剂量复方样品具有清咽作用,且益生菌草本高剂量复方干预致炎剂诱导的急性炎症效果优于高剂量草本。

Objective To explore the throat-clearing effect of the combination of probiotics and herbal extract.Methods The experimental rats were administrated intragastrically probiotics and/or herbal extracts while the control rats was given with water instead for 45 d.The cotton ball implantation test in rats,toe swelling test in rats,and ear swelling test in mice were performed according to the "Test Method of Throat-clearing Effect for Health Food" revised in 2012.Results In the cotton ball implantation test,the net amount of granulomas in the groups of high dose probiotic,medium or high dose herb,low dose probiotics mixed with medium or high dose herb,high dose probiotics mixed with low or medium or high dose herb were significantly lower than the control group(P<0.05),which had the effect of interfering with the formation of granuloma(chronic inflammation).In the toe swelling test,the toe swelling rate in the high dose herb group was significantly reduced after 1 h of inflammatory agent injection(P<0.05).After 1,2,6 h of inflammatory agent injection,the toe swelling rates in high dose probiotics mixed with high dose herb group was significantly lower than those in the control group(P<0.05) and also lower than those in the high dose probiotics group and high dose herb group,suggesting that the compound of high dose probiotics and herb extracts interfered with the toe swelling induced by the inflammatory agent and the reducing-effect was better than those in the high dose probiotics and high dose herb extracts groups;In ear swelling test,there were no significant difference between each group mice on the swelling value and swelling rate of auricle.Conclusion The samples of high dose herbal extracts group and high dose probiotics mixed with high dose herbal extracts group have the throat-clearing effect,and the effect of the combination is better than herbal extracts alone in the intervention of acute inflammation induced by inflammatory agents.