POLE2对非小细胞肺癌细胞侵袭迁移能力的影响

Effect of overexpression of POLE2 on invasion and migration of non-small cell lung cancer cells

目的探讨POLE2对非小细胞肺癌(NSCLC)细胞侵袭迁移能力的影响。方法采用实时荧光定量PCR(qRT-PCR)检测人支气管上皮样细胞BEAS-2B和NSCLC细胞A549、SPC-A1中POLE2的表达水平。构建POLE2过表达细胞模型,qRT-PCR和蛋白质印迹法(Western blot)验证转染效率。采用Cell Counting Kit-8(CCK-8)实验检测NSCLC细胞的增殖能力,Transwell实验检测NSCLC细胞的侵袭迁移能力,qRT-PCR检测MMP9的表达水平。结果人NSCLC细胞SPC-A1、A549中POLE2的表达水平显著高于人支气管上皮样细胞BEAS-2B(P<0.05)。过表达POLE2促进了A549细胞的增殖和侵袭迁移,上调了MMP9的表达(P<0.05)。结论过表达POLE2可能通过上调MMP9促进NSCLC细胞的侵袭迁移。

Objective To investigate the effect of POLE2 on the invasion and migration of non-small cell lung cancer(NSCLC)cells.Methods The expression of POLE2 in human NSCLC cells BEAS-2B and A549,human bronchial epithelial cell line SPC-A1 was detected by real-time PCR(qRT-PCR).The POLE2-overexpression cell was established.The transfection efficiency was verified by qRT PCR and Western blot.Cell counting kit-8(CCK-8)was used to detect the proliferation of NSCLC cells,Transwell was used to detect the invasion and migration of NSCLC cells,and qRT-PCR was used to detect the expression of MMP9.Results The expression level of POLE2 in human NSCLC cells SPC-A1 and A549 was significantly higher than that of BEAS-2B(P<0.05).Overexpression of POLE2 promoted the proliferation,invasion and migration of A549 cells,and upregulated the expression of MMP9(P<0.05).Conclusion Overexpression of POLE2 may promote the invasion and migration of NSCLC cells by up regulating MMP9.