臭氧大自血法对睡眠剥夺大鼠褪黑素及氧化应激指标的影响

Effects of Ozonated Autohemotherapy on Melatonin and Oxidative Stress in Rats with Sleep Deprivation

目的观察臭氧大自血法对睡眠剥夺大鼠褪黑素及氧化应激指标的影响。方法选取40只成年雄性Wistar大鼠,采用"改良多平台睡眠剥夺法"制作快速眼球运动(rapid eye movement sleep, REM)睡眠剥夺模型。根据随机数字表原则,将大鼠分为对照组和观察组,每组各20只。观察组大鼠给予臭氧自体血回输,对照组大鼠以空气进行对照。分别在干预0 h、24 h、48 h和72 h时,采尾静脉血检测血清褪黑素水平,在干预0 h、72 h时,检测静脉血氧化应激指标超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-px)和丙二醛(MDA)水平。结果干预0 h时,两组大鼠觉醒期(wake, W)、慢波睡眠(slow-wave sleep, SWS)、快波睡眠(fast-wave sleep, FWS)比较,差异均无统计学意义(P>0.05);干预72 h时,观察组大鼠W低于对照组,SWS、FWS高于对照组(P<0.05)。干预48 h、72 h时,观察组大鼠血清褪黑素水平均高于对照组(P<0.05)。干预72 h时,观察组大鼠SOD水平、MDA水平低于对照组,GSH-px水平高于对照组(P<0.05)。结论臭氧大自血法可促进睡眠剥夺大鼠褪黑素释放,降低大鼠血清SOD水平、MDA水平,提高大鼠血清GSH-px水平。

Objective To observe the effects of ozone major autohemotherapy on melatonin and oxidative stress in rats with sleep deprivation,and to provide theoretical basis for the treatment of ozone major autohemotherapy.Methods Forty adult male Wistar rats were selected as the study subjects.A rapid eye movement(REM)sleep deprivation model was subsequently developed using the"modified multi-platform sleep deprivation method".Rats were randomly divided into the control group and the observation group according to the principle of random number table,with 20 rats in each group.The rats in the observation group were given autologous blood transfusion after ozone treatment,and the control rats were given air instead of ozone.Serum melatonin levels were measured at tail vein blood at 0 h,24 h,48 h and 72 h after intervention.The venous blood oxidative stress index[superoxide dismutase(SOD),glutathione peroxidase(GSH-px)and malondialdehyde(MDA)]levels were measured at 0 h and 72 h after intervention.Results There was no significant difference in wake(W),slow-wave sleep(SWS),and fast-wave sleep(FWS)time between the two groups of rats at the 0 h intervention(P>0.05).At 72 hours of intervention,W time in the observation group was shorter than that in the control group,and SWS and FWS time were longer than those in the control group.The differences were statistically significant(P<0.05).At 48 h and 72 h after intervention,the serum melatonin levels in the observation group were higher than those in the control group(P<0.05).At 72 h after intervention,the levels of SOD and MDA in the observation group were lower than those in the control group,and the GSH-px level was higher than that in the control group(P<0.05).Conclusion Ozone major autohemotherapy can promote melatonin release in rats with sleep deprivation,decrease serum SOD level and MDA level,and increase serum GSH-px level in rats.