非小细胞肺癌肿瘤微环境中免疫细胞特征及临床意义

Characteristics and clinical significance of immune cells in tumor microenvironment of non-small cell lung cancer

目的检测非小细胞肺癌(Non-small cell lung cancer,NCSLC)患者肿瘤组织与癌旁组织中各免疫细胞比率,并探讨其与临床病理特征的关系。方法采用流式细胞分析技术检测69例NSCLC患者肿瘤组织及癌旁组织中Treg、TIL、MDSC等免疫细胞比率,通过统计学软件分析其与临床病理特征的相关性。结果 NCSLC患者肿瘤组织中CD+4 T细胞、CD+8 T细胞比率、Treg占总T细胞及Treg细胞占总CD+4 T细胞比率、TIL细胞比率、M-MDSC细胞比率均高于癌旁组织(P<0.01);NCSLC患者肿瘤组织中G-MDSC占总细胞比值与癌旁组织结果无差异,不具有统计学意义(P>0.05)。NCSLC患者肿瘤组织中Treg细胞占总T细胞的比率、TIL细胞、G-MDSC细胞、M-MDSC细胞、Macrophages细胞、CD163+Macrophages(M2)细胞比率与患者性别、年龄、肿瘤病理类型、肿瘤分期、肿瘤大小以及肿瘤是否伴有淋巴结转移均无关,差异无统计学意义(P>0.05)。NCSLC患者肿瘤组织中Treg细胞占总CD+4 T细胞的比值与肿瘤病理类型、肿瘤分期、肿瘤大小以及肿瘤是否伴有淋巴结转移均无关,差异无统计学意义(P>0.05)。NCSLC患者肿瘤组织中Treg细胞占总CD+4 T细胞的比值与患者性别、年龄有关,其中男性患者高于女性患者,大于等于60岁患者高于小于60岁患者,差异具有统计学意义(P<0.05)。结论 NSCLC患者肿瘤微环境中各免疫细胞共同参与肿瘤的发生发展,并促进肿瘤的侵袭与转移。

Objective To detect the ratio of immune cells in tumor and paracancer tissues of patients with non-small cell lung cancer(NCSLC),and to explore its relationship with clinicopathological characteristics.Methods The percentage of immune cells in tumor tissues and adjacent tissues of 69 NSCLC patients including Treg,TIL and MDSC was detected by flow cytometry,and the correlation with clinicopathological features was analyzed by statistical software.Results The ratio of CD+4 T cells,CD+8 T cells,Tregs to total T cells and Tregs to total CD+4 T cells,TIL cells,and M-MDSC cells in NCSLC patients'tumor tissues were higher than those in adjacent tissues(P<0.01).The ratio of G-MDSC to total cells in tumor tissues of NCSLC patients was not different from that of adjacent tissues(P>0.05).The ratio of Treg cells to total T cells in tumor tissues of patients with NCSLC,TIL cells,G-MDSC cells,M-MDSC cells,Macrophages cells,CD163+Macrophages(M2)cell ratio showed no correlation with gender,age,tumor pathological type and tumor stage.There was no relationship between tumor size and whether the tumor was associated with lymph node metastasis,and the difference was not statistically significant(P>0.05).The ratio of Treg cells to total CD+4 T cells in tumor tissues of patients with NCSLC was not related to tumor pathological type,tumor stage,tumor size,and whether the tumor was associated with lymph node metastasis,and the difference was not statistically significant(P>0.05).The ratio of Treg cells to total CD4+T cells in tumor tissues of patients with NCSLC was related to the gender and age of the patients.Among them,male patients were higher than female patients,patients aged 60 years or older were higher than patients aged less than 60 years,and the difference was statistically significant(P<0.05).Conclusion The immune cells in tumor microenvironment of NSCLC patients participate in the development of tumor and promote the invasion and metastasis of tumor.