大疱性类天疱疮患者血清及疱液中SOCS-1和SOCS-3的水平检测

Levels of SOCS-1 and SOCS-3 in serum and bullous fluid of the patients with bullous pemphigoid

目的:检测大疱性类天疱疮(BP)患者血清及疱液中细胞因子信号传导抑制蛋白1(SOCS-1)、细胞因子信号传导抑制蛋白3(SOCS-3)的表达水平。方法:收集我院2017年1月至2018年12月的大疱性类天疱疮患者,同时选取II度烫伤患者为对照组,ELISA法检测患者与对照血清及疱液中SOCS-1、SOCS-3表达水平,自身免疫性疱病严重程度评分(ABSIS)判定患者疾病严重程度。采用Pearson相关性分析法分析BP患者血清与疱液中SOCS-1、SOCS-3表达的关系及SOCS-1、SOCS-3水平与ABSIS的关系。结果:共收集42例BP患者和30例对照。BP患者血清SOCS-1和SOCS-3水平为62.14±12.45和47.23±10.72,高于对照组的31.25±9.85和20.62±9.06,差异具有统计学意义(均P<0.05);BP患者疱液中SOCS-1和SOCS-3水平为106.45±30.27和91.42±24.58,高于对照组的67.26±21.33和51.07±15.16,差异具有统计学意义(均P<0.05);BP患者SOCS-1与SOCS-3水平呈正相关(P<0.05),SOCS-1和SOCS-3水平分别与ABSIS评分呈正相关(P<0.05)。结论:大疱性类天疱疮患者血清及疱液中SOCS-1和SOCS-3表达水平升高,其表达水平与ABSIS评分呈正相关,可能与大疱性类天疱疮疾病的发病机制有关。

Objective:To detect the expression levels of suppressor of cytokine signaling 1(SOCS-1)and suppressor of cytokine signaling 3(SOCS-3)in the serum and bullous fluid of the patients with bullous pemphigoid(BP).Methods:The patients with BP diagnosed in our hospital from January 2017 to December 2018 and the patients with second degree scald used as controls were collected.The expression levels of SOCS-1 and SOCS-3 in the serum and blister fluid of the patients and controls group were detected by EILSA and the severity of BP was assessed through autoimmune blister severity score(ABSIS).The relationship between the expressions of SOCS-1 and SOCS-3 and between the levels of SOCS-1 or SOCS-3 and ABSIS were analyzed by Pearson correlation analysis.Results:Fouty-two patients and 30 patients were collected.The expression levels of SOCS-1 and SOCS-3 in the patients serum were 62.14±12.45 and 47.23±10.72,which were significantly higher than those in the control group(31.25±9.85 and 20.62±9.06)(P s<0.05).The expression levels of SOCS-1 and SOCS-3 in the patients blister fluid were 106.45±30.27 and 91.42±24.58,which were significantly higher than those in the control group 67.26±21.33 and 51.07±15.16(P<0.05).SOCS-1 level was positively correlated with SOCS-3 level in the BP patients(P<0.05).The levels of SOCS-1 and SOCS-3 were positively correlated with ABSIS score respectively(P<0.05).Conclusion:The expression levels of SOCS-1 and SOCS-3 in serum and blister fluid of patients with bullous pemphigoid are up-regulated.The expression levels of SOCS-1 and SOCS-3 are positively correlated with ABSIS scores,which may be associated to the pathogenesis of BP.