慢性乙型肝炎患者血清颗粒蛋白前体与血清T淋巴细胞亚群及炎症因子的相关性

Correlation between serum granulin precursor and serum T lymphocyte subsets and inflammatory factors in patients with chronic hepatitis B

目的研究血清颗粒蛋白前体在慢性乙型肝炎(CHB)患者中的表达,及其与患者血清T淋巴细胞亚群及炎症因子的相关性。方法80例CHB患者纳入研究组,同期体检健康者80例纳入对照组,比较两组血清颗粒蛋白前体的表达。分析不同血清颗粒蛋白前体表达水平与血清T淋巴细胞亚群、炎症因子的相关性。结果颗粒蛋白前体在研究组血清中的表达较对照组明显升高(t=6.57,P<0.05);且随着HBV DNA载量的升高,血清颗粒蛋白前体的表达越高(F=14.23,P<0.05);血清颗粒蛋白前体诊断CHB的截断值为45.32μg/ml,灵敏度为87.69%,特异度为92.14%;血清颗粒蛋白前体表达与T淋巴细胞亚群CD^3+、CD^4+的比率呈负相关关系(r分别=-0.56、-0.68,P均<0.05);与T淋巴细胞亚群CD^8+的比率以及血清炎症因子白介素1β(IL-1β)、白介素6(IL-6)、肿瘤坏死因子α(TNF-α)的表达呈正相关(r分别=0.55、0.42、0.48、0.37,P均<0.05)。结论颗粒蛋白前体在CHB患者血清中呈高表达,提示患者免疫功能的下降以及不良炎症反应。

Objective To study the expression of serum granulin precursor in patients with chronic hepatitis B and its correlation with serum T lymphocyte subsets and inflammatory factors.Methods Totally 80 patients with chronic hepatitis B were included in the study group,and the 80 cases who received examination were enrolled in the control group.The expression of serum granulin precursor was compared between two groups and the correlations between granulin precursor and serum T lymphocyte subsets and inflammatory factors were analyzed.Results The expression of granulin precursor in study group was significantly higher than that in the control group(t=6.57,P<0.05).With the increasing of HBV DNA load,the expression of serum granulin precursor was gradually higher(F=14.23,P<0.05).The cut-off value of serum granulin precursor for diagnosing chronic hepatitis B was 45.32μg/ml,the sensitivity was 87.69%,and the specificity was 92.14%.The expression of serum granulin precursor was negatively correlated with the ratios of T lymphocyte subsets CD^3+and CD^4+(r=-0.56,-0.68,P<0.05),and positively correlated with the ratio of T lymphocyte subsets CD^8+,IL-1β,IL-6,and TNF-α(r=0.55,0.42,0.48,0.37,P<0.05).Conclusion High expression of granulin precursor in the serum of chronic hepatitis B patients indicate that the decline of immune function and adverse inflammatory reaction in patients with hepatitis B.