miR-655-3p通过靶向抑制ZEB1对头颈鳞状细胞癌细胞增殖及侵袭能力的影响

Effects of miR-655-3p on the Cellular Proliferation and Invasion of Head and Neck Squamous Cell Carcinoma Through ZEB1 Targeted Inhibition

目的:研究miR-655-3p在头颈鳞状细胞癌(HNSCC)组织及细胞系中的表达及其对细胞系生物学功能的调控作用。方法:通过qRT-PCR的方法检测HNSCC组织及细胞系(FaDu、SCC-9、TU686、TU212)中miR-655-3p的表达水平,并通过一系列细胞生物学实验检测miR-655-3p对细胞增殖、迁移及侵袭能力的影响,进一步筛选miR-655-3p可能靶基因,探究其作用机制。结果:与癌旁正常组织中表达水平(3.17±0.20)相比,miR-655-3p在HNSCC癌组织中表达降低(2.89±0.17),P<0.05;与对照正常细胞系相比,miR-655-3p在HNSCC细胞系中表达降低,P<0.05。而过表达细胞系中miR-655-3p的表达后,可明显抑制HNSCC细胞系的增殖、迁移及侵袭能力,进一步机制研究表明ZEB1作为miR-655-3p的靶基因受其负向调控,miR-655-3p通过靶向抑制ZEB1的表达进而抑制HNSCC细胞系上皮-间充质转化进程。结论:miR-655-3p在HNSCC癌组织及细胞系中低表达,能够通过靶向调控ZEB1的表达,抑制细胞系恶性表型的进展。

Objective:To study the expression of miR-655-3p in head and neck squamous cell carcinoma(HNSCC)tissues and cell lines and its effects on the biological functions of HNSCC cell lines.Methods:The expression levels of miR-655-3p in HNSCC tissues and cell lines(FaDu,SCC-9,TU686,TU212)were measured by qRT-PCR,and the effects of miR-655-3p on cellular proliferation,migration and invasion were evaluated by a series of assays.The possible target gene of miR-655-3p was further screened and its mechanism was explored.Results:The expression level of miR-655-3p in HNSCC was lower than that in control group,P<0.05.And the over-expression of miR-655-3p in HNSCC cell lines significantly inhibited the proliferation,migration and invasion of cell lines.Further mechanism studies showed that ZEB1,as the target gene of miR-655-3p,was negatively regulated by miR-655-3p.Even,miR-655-3p inhibited the epithelial mesenchymal transformation of HNSCC cell lines by targeting ZEB1 expression directly.Conclusion:MiR-655-3p is down-regulated in HNSCC cancer tissues and cell lines,which could target the expression of ZEB1 directly and inhibit the progression of malignant phenotype of cell lines.